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Nitrification in coniferous forest soils

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Abstract

Net nitrification rates tend to be low or negligible in the forest floor of many coniferous forests of North-East Scotland. The most likely process controls are substrate availability, pH, allelopathy, water potential, nutrient status and temperature. These are discussed in relation to field and laboratory studies of net and potential rates of nitrification.

Fungi make up by far the largest part of the nitrifier community in the coniferous forest floor. Very little is known about the distribution and activity of autotrophs in these systems, although it is certain that in vitro evidence suggesting autotrophs cannot nitrify at pH levels characteristic of coniferous forest soils is unrealistic.

Because of the metabolic diversity of nitrifying fungi, a variety of organic and inorganic nitrification pathways may exist in coniferous forests. The possible involvement of free radicles in fungal nitrification in coniferous forest soils is also suggested.

A complete understanding of nitrification in coniferous forest soils can only result from field characterisation of N flux such as through the use of 15N. This must be combined with ecophysiological characterisation of the organisms involved in order that the complexity of nitrification in coniferous forest soils can be resolved.

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References

  • Adams J A 1986a Nitrification and ammonification in acid forest litter and humus as affected by peptone and ammonium-N amendment. Soil Biol. Biochem. 18, 45–51.

    Google Scholar 

  • Adams J A 1986b Identification of heterotrophic nitrification in strongly acid larch humus. Soil Biol. Biochem. 18, 339–341.

    Google Scholar 

  • Aleem M I H 1975 Biochemistry reaction mechanisms in sulphur oxidation by chemosynthetic bacteria. Plant and Soil 43, 587–607.

    Google Scholar 

  • Aleem M I H, Lees H and Lyric R 1964 Ammonium oxidation by cell-free extracts of Aspergillus wentii. Can. J. Biochem. 42, 989–998.

    Google Scholar 

  • Baath E, Berg B, Lohm U, Lundgren B, Lundkvist H, Söderström B and Wirén A 1980 Effects of experimental acidification and liming on soil organisms and decomposition in a Scots pine forest. Pedobiologia 20, 85–100.

    Google Scholar 

  • Bartlett R J 1981 Nonmicrobial nitrite-to-nitrate transformation in soils. Soil Sci. Soc. Am. J. 45, 1054–1058.

    Google Scholar 

  • Becker G E 1967 Effect of glucose on the oxidation of beta-nitropropionic acid by Aspergillus flavus. J. Bacteriol. 94, 48–52.

    Google Scholar 

  • Berg P, Klemedtsson L and Rosswall T 1982 Inhibitory effect of low partial pressures of acetylene on nitrification. Soil Biol. Biochem. 14, 301–303.

    Google Scholar 

  • Bruehl G W, Sprague R, Fischer W R, Nagamitsu M, Nelson W L and Vogel O H 1981 Washington Agricultural Experimental Station Bulletin 677, 1–21.

  • Cooper J E 1975 Nitrification in soils incubated with pig slurry. Soil Biol. Biochem. 7, 119–124.

    Google Scholar 

  • Cutler D W and Mukerji B K 1931 Nitrite formation by soil bacteria other than Nitrosomonas. Proc. R. Soc. 108, Series B, 384–394.

    Google Scholar 

  • De Boer W, Duyts H and Laanbroek H J 1989 Urea stimulated autotrophic nitrification in suspensions of a fertilized, acid heath soil. Soil Biol. Biochem. 21, 349–354.

    Google Scholar 

  • Doxtader K G 1965 Nitrification by Heterotrophic Microorganisms. Ph.D. Thesis, Cornell University, Ithaca, NY.

  • Doxtader K G and Alexander M 1966 Nitrification by heterotrophic soil micro-organisms. Soil Sci. Soc. Am. Proc. 30, 351–355.

    Google Scholar 

  • Dubey H D 1968 Effect of soil moisture on nitrification. Can. J. Microbiol. 14, 1348–1350.

    Google Scholar 

  • Edwards A C and Killham K 1986 The effect of freeze-thaw on gaseous nitrogen loss from upland soils. Soil Use Manag. 2, 86–91.

    Google Scholar 

  • Eylar O R and Schmidt E L 1959 A survey of heterotrophic microorganisms from soil for ability to form nitrite and nitrate. J. Gen. Microbiol. 20, 473–481.

    Google Scholar 

  • Gowder T K S, Siddaramappa R and Sethunathan N 1976 Heterotrophic nitrification and nitrite tolerance by Aspergillus carneus, a predominant fungus isolated from benomyl-amended soil. Soil Biol. Biochem. 8, 435–437.

    Google Scholar 

  • Griffin D M 1969 Soil water and the ecology of fungi. Annu. Rev. Phytopathol. 15, 319–329.

    Google Scholar 

  • Griffin D M 1977 Water potential and wood decay fungi. Annu. Rev. Phytopathol. 15, 319–329.

    Google Scholar 

  • Hankinson T R and Schmidt E L 1984 Examination of an acid forest soil for ammonia- and nitrite-oxidizing autotrophic bacteria. Can. J. Microbiol. 30, 1125–1132.

    Google Scholar 

  • Harmer R and Alexander I 1986 The effect of starch amendment on nitrogen mineralisation from the forest floor beneath a range of conifers. Forestry 59, 39–46.

    Google Scholar 

  • Hatcher J H and Schmidt E L 1971 Nitrification of aspartate by Aspergillus flavus. Appl. Microbiol. 21, 181–186.

    Google Scholar 

  • Heilman P 1974 Effect of urea fertilization on nitrification in forest soils of the Pacific North West. Soil Sci. Soc. Am. Proc. 38, 664–667.

    Google Scholar 

  • Hirsch P L, Overrein L N and Alexander M 1961 Formation of nitrite and nitrate by actinomycetes and fungi. J. Bacteriol. 82, 442–448.

    Google Scholar 

  • Hynes R K and Knowles R 1982 Effect of acetylene on autotrophic and heterotrophic nitrification. Can. J. Microbiol. 28, 334–340.

    Google Scholar 

  • Ishaque M and Sarker M S I 1978 Production of nitrate during incubation of inoculated, autoclaved tea soil by a strain of isolated Aspergillus spp. Bangladesh J. Scientific Res. 1 (A & B), 31–39.

    Google Scholar 

  • Johnson D W, Edwards N T and Todd D E 1980 Nitrogen mineralization, immobilization and nitrification following urea fertilization of a forest soil under field and laboratory conditions. Soil Sci. Soc. Am. J. 44, 610–616.

    Google Scholar 

  • Johnsrud S C 1978 Heterotrophic nitrification in acid forest soils. Holarctic Ecol. 1, 27–30.

    Google Scholar 

  • Kaila A 1954 Nitrification in decomposing organic matter. Acta Agric. Scand. 4, 17–32.

    Google Scholar 

  • Kapralek F 1962 The physiology of riboflavin production by Eremothecium ashbyi. J. Gen. Microbiol. 29, 403–419.

    Google Scholar 

  • Killham K 1987a A new perfusion system for the measurement and characterization of potential rates of soil nitrification. Plant and Soil 97, 267–272.

    Google Scholar 

  • Killham K 1987b Heterotrophic nitrification. In Nitrification. Ed. J Prosser. pp 117–126. SGM Special Publication.

  • Killham K 1990 Soil Ecology. Chapman Hall (In press).

  • King J A, Smith K A and Pyatt G D 1986 Water and oxygen regimes under conifer plantations and native vegetation on upland peaty grey soil and deep peat soils. J. Soil Sci. 37, 485–497.

    Google Scholar 

  • Liang C N and Tabatabai M A 1977 Effects of trace elements on nitrogen mineralisation in soils. Environ. Pollut. 12, 141–147.

    Google Scholar 

  • Likens G E, Borman F H and Johnson N M 1969 Nitrification: Importance to nutrient losses from a cutover forested ecosystem. Science 163, 1205–1206.

    Google Scholar 

  • Lodhi M A K 1977 The influence and comparison of individual forest trees on soil properties and possible inhibition of nitrification due to intact vegetation. Am. J. Bot. 64, 260–264.

    Google Scholar 

  • Lodhi M A K 1978 Comparative inhibition of nitrifiers and nitrification in a forest community as a result of the allelopathic nature of various tree species. Am. J. Bot. 65, 1135–1137.

    Google Scholar 

  • Malhi S S and McGill W B 1982 Nitrification in three Alberta soils: Effect of temperature, moisture and substrate concentration. Soil Biol. Biochem. 14, 393–399.

    Google Scholar 

  • Martikainen P J 1984 Nitrification in two coniferous forest soils after different fertilization treatments. Soil Biol. Biochem. 16, 577–582.

    Google Scholar 

  • Martikainen P J 1985 Numbers of autotrophic nitrifiers and nitrification in fertilized forest soil. Soil Biol. Biochem. 17, 245–248.

    Google Scholar 

  • Martikainen P J and Nurmiaho-Lassila E L 1985 Nitrosospira, an important ammonium-oxidizing bacterium in fertilized coniferous forest soil. Can. J. Microbiol. 31, 190–197.

    Google Scholar 

  • Molina J A E and Alexander M 1971 Formation of nitrate from 3-nitropropionate by Aspergillus flavus. J. Bacteriol. 105, 489–493.

    Google Scholar 

  • Nelson D H 1929 The isolation of some nitrifying organisms. Iowa State Coll. J. Sci. 3, 113–175.

    Google Scholar 

  • Nommik H 1978 Mineralization of carbon and nitrogen in forest humus as influenced by additions of phosphate and lime. Acta Agric. Scand. 28, 221–230.

    Google Scholar 

  • Odu C T I and Adeoye K B 1970 Heterotrophic nitrification in soils—A preliminary investigation. Soil Biol. Biochem. 2, 41–45.

    Google Scholar 

  • Overrein L N 1971 Isotope studies on nitroge in forest soil. 1. Relative losses of nitrogen through leaching for forty months. Rep. Norw. For. Res. Inst. 114, 261–280.

    Google Scholar 

  • Painter H A 1970 A review of literature on inorganic nitrogen metabolism in microorganisms. Water Res. 4, 393–450.

    Google Scholar 

  • Popovic B 1977 Effect of ammonium nitrate and urea fertilizers on nitrogen mineralization, especially nitrification, in a forest soil. Royal College of Forestry, Stockholm. Departments of Forest Ecology and Forest Soils. Research Notes 30, 1–33.

    Google Scholar 

  • Pyatt D G and Smith K A 1983 Water and oxygen regimes of four soil types at Newcastleton Forest, South Scotland. J. Soil Sci. 37, 485–497.

    Google Scholar 

  • Remacle J 1977a The role of heterotrophic nitrification in acid forest soils—preliminary results. Ecol. Bull. (Stockholm) 25, 560–561.

    Google Scholar 

  • Remacle J 1977b Microbial transformations of nitrogen in forests. Oecol. Plant. 7, 69–78.

    Google Scholar 

  • Rice E L and Pancholy S K 1972 Inhibition of nitrification by climax vegetation. Am. J. Bot. 59, 1033–1040.

    Google Scholar 

  • Rice E L and Pancholy S K 1974 Inhibition of nitrification by climax ecosystems. III. Inhibitors other than tannins. Am. J. Bot. 61, 1095–1103.

    Google Scholar 

  • Roberge M R and Knowles R 1966 ureolysis, immobilization, and nitrification in Black spruce (Picea mariana Mill) humus. Soil Sic. Soc. Am. Proc. 30, 201–204.

    Google Scholar 

  • Russell E J and Russell E W 1961 Soil Conditions and Plant Growth. 9th ed. Wiley, New York.

    Google Scholar 

  • Schimel J P, Firestone M K and Killham K 1984 Identification of heterotrophic nitrification in a Sierra forest soil. Appl. Environ. Microbiol 48, 802–806.

    Google Scholar 

  • Schmidt E L 1954 Nitrate formation by a soil fungus. Science 119, 187–189.

    Google Scholar 

  • Schmidt E L 1960 Cultural conditions influencing nitrate formation by Aspergillus flavus J. Bacteriol. 79, 553–557.

    Google Scholar 

  • Smith W, Borman F H and Likens G E 1968 Response of chemoautotrophic nitrifiers to forest cutting. Soil Sci. 106, 471–473.

    Google Scholar 

  • Tate R L 1977 Nitrification in histosols: A potential role for the heterotrophic nitrifier. Appl. Environ. Microbiol. 33, 911–914.

    Google Scholar 

  • Van Gool H J and Schmidt E L 1973 Nitrification in relation to growth in Aspergillus flavus. Soil Biol. Biochem. 5, 259–265.

    Google Scholar 

  • Van Praag H J and Weissen F 1973 Elements of a functional defintion of oligotroph humus based on the nitrogen nutrition of forest stands. J. Appl. Ecol. 10, 569–583.

    Google Scholar 

  • Verstraete W 1975 Heterotrophic nitrification in soils and aqueous media—a review. Bull. Academ. Science, U.S.S.R. Biology Series 4, 515–530.

    Google Scholar 

  • Viro P J 1962 Factorial experiments on forest humus decomposition. Soil Sci. 95, 24–30.

    Google Scholar 

  • Vitousek P M, Gosz J R, Grier C C, Melillo J M and Reiners W A 1982 A comparative analysis of potential nitrification and nitrate mobility in forest ecosystems. Ecol. Monogr. 52, 155–177.

    Google Scholar 

  • Waid J S 1975 Hydroxamic acids in soil systems. In Soil Biochemistry. Eds. E A Paul and A D McLaren. Volume 4. New York: Marcel Dekker.

    Google Scholar 

  • White J P and Johnson G T 1982 Aflatoxin production correlation with nitrification in Aspergillus flavus group species. Mycologia 74, 718–723.

    Google Scholar 

  • Williams B L 1972 Nitrogen mineralisation and organic matter decomposition in Scots pine humus. Forestry 45, 177–188.

    Google Scholar 

  • Williams B L 1983 Nitrogen transformations and decomposition in litter and humus from beneath closed canopy Sitka spruce. Forestry 56, 17–32.

    Google Scholar 

  • Wood P M 1988 Mechanisms for biological ammonia oxidation. In The Nitrogen and Sulphur Cycles. Eds. J A Cole and S Ferguson. pp 219–243. SGM Special Publication, Cambridge University Press.

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  1. Department of Plant and Soil Science, University of Aberdeen, AB9 2UE, Aberdeen, Scotland, UK

    K. Killham

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Killham, K. Nitrification in coniferous forest soils. Plant Soil 128, 31–44 (1990). https://doi.org/10.1007/BF00009394

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