Summary
Several Bacillus strains, from the rhizosphere of Ammophila arenaria, appeared on ‘nitrogen-free’ agar plates. They were able to grow in nitrogen-poor medium to which 0.1% yeast extract was added. Three of these bacilli were tested for their ability to fix nitrogen using the acetylene reduction assay. The C2H2-reducing activity was determined at 8-hour intervals during their growth cycle. C2H2 reduction (and accordingly N2 fixation) was greater under anaerobic than aerobic conditions. Additions of 0.1% CaCO3 significantly increased the C2H2-reducing activity under both conditions. Characterisation suggests that these strains are new nitrogen-fixing Bacillus species. re]19740121
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Abdel Wahab, A.M. Nitrogen fixation by Bacillus strains isolated from the rhizosphere of Ammophila arenaria . Plant Soil 42, 703–708 (1975). https://doi.org/10.1007/BF00009953
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DOI: https://doi.org/10.1007/BF00009953