Abstract
We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid treatment of embryos precociously induces or enhances accumulation of the mRNA. Nucleotide sequencing studies show that the deduced 30 kDa polypeptide has an unusual primary structure; the polypeptide possesses direct amino acid sequence repeats and is virtually entirely hydrophilic with the exception of a hydrophobic carboxyl-terminal region. Based upon the expression pattern and predicted polypeptide sequence, we conclude that the mRNA is encoded by a late embryogenesis-abundant (Lea) gene in B. napus.
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Abbreviations
- ABA:
-
abscisic acid
- bp:
-
base pairs
- DAF:
-
days after flowering
- HAI:
-
hours after the start of imbibition
- kb:
-
kilobase (pairs)
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Harada, J.J., DeLisle, A.J., Baden, C.S. et al. Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development. Plant Mol Biol 12, 395–401 (1989). https://doi.org/10.1007/BF00017579
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DOI: https://doi.org/10.1007/BF00017579