Abstract
Efficient production of ATP and NADPH by the ‘light’ reactions of oxygen-evolving photosynthesis demands continuous adjustment of transfer of absorbed light energy from antenna complexes to Photosystem I (PS I) and II (PS II) reaction center complexes in response to changes in light quality. Treatment of intact cyanobacterial cells with N-ethylmaleimide appears to disrupt energy transfer from phycobilisomes to Photosystem I (PS I). Energy transfer from phycobilisomes to Photosystem II (PS II) is unperturbed. Spectroscopic analysis indicates that the individual complexes (phycobilisomes, PS II, PS I) remain functionally intact under these conditions. The results are consistent with the presence of connections between phycobiliproteins and both PS II and PS I, but they do not support the existence of direct contacts between the two photosystems.
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Abbreviations
- Chl:
-
chlorophyll
- EPR:
-
electron paramagnetic resonance
- NEM:
-
N-ethylmaleimide
- PBS:
-
phycobilisome
- PS:
-
photosystem
References
Aizawa K, Shimizu T, Hiyama T, Satoh K, Nakamura Y and Fujita Y (1992) Changes in composition of membrane proteins accompanying the regulation of PS I/PS II stoichiometry observed with Synechocystis PCC 6803. Photosynth Res 32: 131–138
Biggins J and Bruce D (1989) Regulation of excitation energy transfer in organisms containing phycobilins. Photosynth Res 20: 1–34
Bryant DA (1987) The cyanobacterial photosynthetic apparatus: comparisons to those of higher plants and photosynthetic bacteria. Can Bull Fish Aquat Sci 14: 423–500
Bryant DA (1991) Puzzles of chloroplast ancestry. Current Biol 2: 240–242
Buchanan BB (1992) Carbon dioxide assimilation in oxygenic and anoxygenic photosynthesis. Photosynth Res 33: 147–162
Clement-Metral JD, Gantt E and Redlinger T (1985) A Photosystem II-phycobilisome preparation from the red alga, Porphyridium cruentum: oxygen evolution, ultrastructure, and polypeptide resolution. Arch Biochem Biophys 238: 10–7
deLorimier R, Bryant DA and Stevens SEJr (1990) Genetic analysis of a 9 kDa phycocyanin-associated linker polypeptide. Biochim Biophys Acta 1019: 29–41
Evans EH (1981) Photosystem I preparations from dark- and light-grown cells of the cyanobacterium Chlorogloea fritschii. Photosynth Res 1: 259–264
Gantt E (1980) Structure and function of phycobilisomes: Light-harvesting pigment complexes in red and blue-green algae. Int Rev Cytol 66: 45–80
Glazer AN (1983) Comparative biochemistry of photosynthetic light-harvesting systems. Ann Rev Biochem 52: 125–157
Glazer AN (1989) Light guides. Directional energy transfer in a photosynthetic antenna. J Biol Chem 264: 1–4
Glazer AN and Melis A (1987) Photochemical reaction centers: structure, organization, and function. Ann Rev Plant Physiol 38: 11–45
Kashino Y, Enami I, Igarashi S and Katoh S (1992) An oxygenevolving Photosystem II complex associated with the core substructure of the phycobilisome from Synechococcus elongatus. Plant Cell Physiol 33: 259–266
Kura-Hotta M, Satoh K and Katoh S (1986) Functional linkage between phycobilisome and reaction center in two phycobilisome oxygen-evolving Photosystem II preparations isolated from the thermophilic cyanobacterium Synechococcus sp. Arch Biochem Biophys 249: 1–7
Ley AC and Butler WL (1980) Energy distribution in the photochemical apparatus of Porphyridium cruentum in State I and State II Biochim Biophys Acta 592: 349–363
Means GE and Feeney RE (1971) Chemical Modification of Proteins, pp. 110–114. Holden-Day Inc, San Francisco
Mullineaux CW (1992) Excitation energy transfer from phycobilisomes to Photosystem I in a cyanobacterium. Biochim Biophys Acta 1100: 285–292
Myers J, Graham J-R and Wang RT (1980) Light-harvesting in Anacystis nidulans studied in pigment mutants. Plant Physiol 66: 1144–1149
Pakrasi HB and Sherman LA (1984) A highly active oxygenevolving Photosystem II preparation from the cyanobacterium Anacystis nidulans. Plant Physiol 74: 742–745
Peter GF and Thornber JP (1991) Biochemical composition and organization of higher plant Photosystem II light-harvesting pigment-proteins. J Biol Chem 266: 16745–16754
Schirmer T, Bode W, Huber R, Sidler W and Zuber H (1985) X-ray crystallographic structure of the light-harvesting biliprotein C-phycocyanin from the thermophilic cyanobacterium Mastigocladus laminosus and its resemblance to globin structures. J Mol Biol 184: 257–277
Schluchter WM and Bryant DA (1992) Molecular characterization of ferredoxin-NADP+ oxidoreductase in cyanobacteria: cloning and sequence of the petH gene of Synechococcus sp. PCC7002 and studies of the gene product. Biochemistry 31: 3092–3102
Siebzehnrübl S, Fischer R and Scheer H (1987) Chromophore assignment in C-phycocyanin from Mastigocladus laminosus. Z Naturforsch 42c: 258–262
Stanier RY, Kunisawa R, Mandel M and Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (Order Chroococcales) Bacteriol Rev 35: 171–205
Webb JL (1966) Enzyme and Metabolic Inhibitors, Vol 3, pp 337–365. Academic Press, New York
Yamanaka GA, Glazer AN and Williams RC (1978) Cyanobacterial phycobilisomes. Characterization of the phycobilisomes of Synechococcus sp. 6301. J Biol Chem 253: 8303–8310
Zhao J, Zhou J and Bryant DA (1992) Energy transfer processes in phycobilisomes as deduced from analyses of mutants of Synechococcus sp. PCC 7002. In: Murata N (ed) Research in Photosynthesis, Vol I, pp 25–32. Kluwer Academic Publishers, Dordrecht, The Netherlands
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Glazer, A.N., Gindt, Y.M., Chan, C.F. et al. Selective disruption of energy flow from phycobilisomes to Photosystem I. Photosynth Res 40, 167–173 (1994). https://doi.org/10.1007/BF00019333
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DOI: https://doi.org/10.1007/BF00019333