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Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens

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Abstract

The molecular basis of complementation by a mixture of two different types of octopine T-region mutants (LBA4060 and LBA4210) was studied. Six randomly chosen cellular clones derived from a tumor obtained after mixed infection were analyzed for their T-DNA content via Southern blot hybridization. The clones appeared to contain T-DNA that originated from each of both mutants, indicating that they developed from doubly infected single cells. Genetic complementation, therefore, might explain at least in part the observed complementation phenomenon. However, complementation as a result of cross-feeding between separately transformed cells could not be excluded. Following protoplast isolation, small aggregates might have formed that developed into the clones analyzed.

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Authors and Affiliations

  1. Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands

    Gert Ooms, Lucy Molendijk & Robbert A. Schilperoort

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  1. Gert Ooms
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  2. Lucy Molendijk
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  3. Robbert A. Schilperoort
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Ooms, G., Molendijk, L. & Schilperoort, R.A. Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens . Plant Mol Biol 1, 217–226 (1982). https://doi.org/10.1007/BF00021033

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  • DOI: https://doi.org/10.1007/BF00021033

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