Summary
The introgression of wildfire (races 0 and 1) and angular leaf spot (ALS) resistance from N. rustica var. Brasilea into N. tabacum has proved economically useful in Zimbabwe although the mode of inheritance of, and genetic relationships between the resistance are unknown. This study was undertaken to (1) examine the mode of inheritance of the resistance to races 0 and 1 of wildfire, and ALS, (2) determine the genetic relationship between the resistances and (3) establish whether the N. rustica-derived wildfire race 0 resistance is allelic to that obtained from N. longiflora. Inheritance was examined under greenhouse and field conditions by studying disease reactions in the parental, F1, F2 and backcross generations derived from crosses of three susceptible lines to a resistant line Nr-7. Three-point backcrosses to the susceptible parent were examined for linkage and segregating generations from a cross of Nr-7 to Burley 21 which carries the N. longiflora race 0 resistance were used to test for allelism. In general, we observed that all resistances are determined by a single dominant gene although some incosistent ratios were obtained likely due to misclassification of disease reactions and erratic transmission. All resistances showed linkage although pleiotropism cannot be ruled out. Allelism tests demonstrated that the N. rustica race 0 resistance is not allelic to that obtained from N. longiflora. Our findings are examined in relation to the efficacy of indirect selection for resistance.
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References
Clayton E.E., 1947. A wildfire resistant tobacco. J. Hered. 38: 35–40.
Clayton E.E., H.E. Heggestad, J.G. Gross, D.R. Bowman & E.O. Schneider, 1951. Breeding behaviour and growth responses resulting from the transfer of wildfire resistance from N. longiflora to N. tabacum. Phytopathology 41: 7.
Deall M.W. & J.S. Cole, 1986. A comparative study of the pathogenicity and epidemiology of strains of Pseudomonas syringae pv. tabaci that cause wildfire and angular leaf spot diseases of tobacco in Zimbabwe. Plant Pathol. 35: 74–81.
Diachun S. & J. Troutman, 1954. Multiplication of Pseudomonas tabaci in leaves of burley tobacco, Nicotiana longiflora and hybrids. Phytopathology 44: 186–187.
Fulton R.W., 1980. Tobacco blackfire disease in Wisconsin. Plant Dis. 64: 100.
Heggestad, H.E., E.E. Clayton, M.O. Neas & H.A. Skoog, 1960. Development of Burley 21, the first wildfire-resistant variety, including variety trials. Tenn. Agric. Exp. Stn. Bull. 321.
Knoche K.K., M.K. Clayton & R.W. Fulton, 1984. Comparison of resistance in tobacco to Pseudomonas syringae pv. tabaci races 0 and 1 by infectivity titrations and bacterial multiplication. Phytopathology 77: 1364–1368.
Knott D.R. & J. Dvorak, 1976. Alien germplasm as a source of resistance to disease. Ann. Rev. Phytopathol. 14: 211–235.
Lucas G.B., 1975. Diseases of tobacco, 3rd ed. Biological Consulting Associates, Raleigh, NC.
Lyle J.C.F., R.A.F. Ternouth, J.J. Woodend & A.E. Matibiri, 1988. Breeding for resistance for wildfire (Pseudomonas syringae pv. tabaci) races 0 and 1, and angular leaf spot (P. syringae pv. angulata). CORESTA Info. Bull 1988: 139–140 (Abst.).
Rufty R.C., E.A. Wernsman & G.V. GoodingJr., 1987. Use of detached leaves to evaluate tobacco haploids and doubled haploids for resistance to tobacco mosaic virus, Meloidogyne incognita and Pseudomonas syringae pv. tabaci. Phytopathology 77: 60–62.
Skoog H.A. & R.W. Fulton, 1976. Sources of resistance in the Nicotiana species to Pseudomonas tabaci virulent on TL 106 derived tobacco. Proc. Am. Phytopath. Soc. 3: 231 (Abst.).
Stavely, J.R., 1979. Disease resistance. p. 87–110. In: R.D. Durbin (Ed). Nicotiana: Procedures for experimental use. USDA Agric. Tech. Bull. 1586.
Stavely J.R. & H.A. Skoog, 1976. Transfer of resistance to a virulent strain of Pseudomonas tabaci from Nicotiana rustica to Nicotiana tabacum breeding lines. Proc. Am. Phytopath. Soc. 3: 231 (Abst.).
Stavely J.R. & H.A. Skoog, 1978. Stabilization in Nicotiana tabacum of a dominant gene from N. rustica for resistance to a virulent strain of Pseudomonas tabaci. Phytopathol. News 12: 181.
Stokes G.W., 1960. Difference in the behaviour of the Nicotiana longiflora wildfire resistance locus in tobacco varieties Burley 21 and KY 61. Phytopathology 50: 770–772.
Stokes G.W., 1963. Wildfire inoculations on burley tobacco. Tob. Sci. VII: 163–165.
Vallleua W.D., C.C. Litton & E.M. Johnson, 1962. Susceptibility of wildfire-resistant tobacco varieties to certain strains of Pseudomonas tabaci and Pseudomonas angulata. Plant Dis. Rep. 46: 36–37.
Wannamaker M.J. & R.C. Rufty, 1989. Development of a method to evaluate tobacco genotypes for resistance to angular leaf spot in the greenhouse. Plant Dis. 73: 964–968.
Wannamaker M.J. & R.C. Rufty, 1990. Inheritance of resistance to angular leaf spot in Nicotiana tabacum L. cultivars Burley 21 and Kentucky 14. Euphytica 46: 252–258.
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Woodend, J.J., Mudzengerere, E. Inheritance of resistance to wildfire and angular leaf spot derived from Nicotiana rustica var. Brasilea. Euphytica 64, 149–156 (1992). https://doi.org/10.1007/BF00023549
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DOI: https://doi.org/10.1007/BF00023549