Summary
Phenotypic stability of bacterial leaf spot resistance in peach (Prunus persica (L.) Batsch) regenerants, either selected at the cellular level for insensitivity to a toxic culture filtrate of Xanthomonas campestris pv. pruni or screened at the whole plant level for resistance to X. campestris pv. pruni, was investigated. A detached-leaf bioassay was used to evaluate the original regenerants again after three years in the greenhouse and also after a two to three year cycle of tissue culture propagation. Peach trees derived through micropropagation from the original regenerants were also evaluated after one to three years growth in the field. Although leaf spot resistance was retained in some regenerants over time in the greenhouse, following in vitro propagation, and under field conditions, resistance was either lost or not expressed in others. Regenerants # 19-1 and #156-6, derived from embryo callus of bacterial spot susceptible ‘Sunhigh’, were significantly more resistant than ‘Sunhigh’. High levels of resistance were exhibited in greenhouse plants and field-grown trees of regenerant #122-1, derived from embryo callus of moderately resistant ‘Redhaven’. This research provides additional evidence that selecting or screening for somaclonal variants with disease resistance is a feasible approach to obtaining peach trees with increased levels of bacterial spot resistance.
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Abbreviations
- TC:
-
Tissue-Cultured
- TF:
-
Toxic culture Filtrate
References
Behnke M., 1979. Selection of potato callus for resistance to culture filtrates of Phytophthora infestans and regeneration of resistant plants. Theor. Appl. Genet. 55: 69–71.
Behnke M., 1980. General resistance to late blight of Solanum tuberosum plants regenerated form callus resistant to culture filtrates of Phytophthora infestans. Theor. Appl. Genet. 56: 151–152.
Chaleff R.S., 1981. Genetics of Higher Plants. Cambridge University Press, Cambridge.
Daub M.E., 1986. Tissue culture and the selection of resistance to pathogens. Annu. Rev. Phytopathol. 24: 159–186.
Hammerschlag F.A., 1982a. Factors affecting establishment and growth of peach shoot tips in vitro. HortScience 17: 85–86.
Hammerschlag F.A., 1982b. Factors influencing in vitro multiplication and rooting of the plum rootstock Myrobalan (Prunus cerasifera Ehrh.). J. Amer. Soc. Hort. Sci. 107: 44–47.
Hammerschlag F.A., 1984. In vitro approaches to disease resistance. In: G.B.Collins & J.G.Petolino (Eds.), Applications of Genetic Engineering to Crop Improvement, pp. 453–490. Martinus Nijhoff/Junk, Dordrecht.
Hammerschlag F.A., 1988. Selection of peach cells for insensitivity to culture filtrates of Xanthomonas campestris pv. pruni and regeneration of resistant plants. Theor. Appl. Genet. 76: 865–869.
Hammerschlag F.A., 1990. Resistance responses of plants regenerated from peach callus cultures to Xanthomonas campestris pv. pruni. J. Amer. Soc. Hort. Sci. 115: 1034–1037.
Hammerschlag F.A. G.Bauchan & R.Scorza, 1985. Regeneration of peach plants from callus derived from immature embryos. Theor. Appl. Genet. 70: 248–251.
Hammerschlag F.A., G.Bauchan & R.Scorza, 1987. Factors influencing in vitro multiplication and rooting of peach cultivars. Plant Cell Tissue & Organ Culture 8: 235–242.
Hartman C.L., T.J.McCoy & T.R.Knous, 1984. Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporum f. sp. medicaginis. Plant Sci. Lett. 34: 183–194.
Heath-Pagliuso S., J.Pullman & L.Rappaport, 1989. ‘UC-T3 somaclone’: Celery germplasm resistant to Fusarium oxysporum f. sp. apii race 2. HortScience 24: 711–712.
Krishnamurthi M. & J.Tlaskal, 1974. Fiji disease resistant Saccharum officinarum var. Pindar subclones from tissue cultures. Proc. Int. Soc. Sugarcane Technol. 15: 130–137.
Larkin P.J. & W.R.Scowcroft, 1981. Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.
Latunde-Dada A.O. & J.A.Lucas, 1988. Somaclonal variation and resistance to Verticilium wilt in lucerne, Medicago sativa L., plants regenerated from callus. Plant Science 58: 111–119.
Lorz H., 1984. Variability in tissue culture derived plants. In: W.Arber (Ed.), Genetic Manipulation; Impact on Man and Society, pp. 103–114. Cambridge Univ. Press, Cambridge.
Matthee F.N. & R.H.Daines, 1968. Effects of soil types and substrate aeration on stomatal activity, water diffusion, pressure deficit, water congestion, and bacterial infection of peach and pepper foliage. Phytopathology 58: 1298–1301.
McCoy T.J., R.L.Phillips & H.W.Rines, 1982. Cytogenetic analysis of plants regenerated from oat (Avena sativa L.) tissue cultures and sectoring in some regenerated plants. Can J. Genet. Cytol 24: 559–565.
Murashige T. & F.Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Randhawa P.S. & E.L.Civerolo, 1985. A detached leaf bioassay for Xanthomonas campestris pv. pruni. Phytopathology 75: 1060–1063.
Ritchie D.F., D.J.Werner & F.A.Hammerschlag, 1993. Field evaluation of tissue culture-derived peach trees for susceptibility to bacterial spot (Xanthomonas campestris pv. pruni. Acta Hort 336: 155–163.
Sacristan M.D., 1982. Resistance responses to Phoma lingam of plants regenerated from selected cell and embryogenic cultures of haploid Brassica napus. Theor. Appl. Genet. 61: 193–200.
Werner D.J., D.F.Ritchie, D.W.Cain & E.I.Zehr, 1986. Susceptibility of peaches and nectarines, plant introductions, and other Prunus species to bacterial spot. HortScience 21: 127–130.
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Hammerschlag, F.A., Werner, D.J. & Ritchie, D.F. Stability of bacterial leaf spot resistance in peach regenerants under in vitro, greenhouse and field conditions. Euphytica 76, 101–106 (1994). https://doi.org/10.1007/BF00024026
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DOI: https://doi.org/10.1007/BF00024026