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Study of the variation in the somatic embryogenesis ability of some pea lines (Pisum sativum L. and Pisum arvense L.)

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Summary

Thirty lines of pea were tested in vitro to evaluate their ability to produce somatic embryos. Three distinct genotypic classes were detected (strong, medium and weak). The best responses were obtained in Pisum sativum. Abnormal somatic embryos and secondary embryogenesis seem to constitute the principal obstacle to the development of these structures.

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References

  • Atanassov A.I. & A.D. Mehandjiev, 1979. In vitro induced morphogenesis in pea. R Acad Bulg Sci 32: 115–118.

    Google Scholar 

  • Bhaskaran S. & R.H. Smith, 1988. Enhanced somatic embryogenesis in Sorghum bicolor (L.) from shoot tip culture. In Vitro Cell Dev Biol 24: 65–70.

    Google Scholar 

  • Bhaskaran S. & R.H. Smith, 1990. Regeneration in cereal tissue culture: A review. Crop Sci 30: 1328–1337.

    Google Scholar 

  • Chee P.P., 1991. Somatic embryogenesis and plant regeneration of squash (Cucurbita pepo L.) cv. YC60. Plant Cell Rep 9: 620–622.

    Google Scholar 

  • Cheng T.Y. & R.H. Smith, 1975. Organogenesis from callus culture of Hordeum vulgare. Planta 123: 307–310.

    Google Scholar 

  • Eapen S. & L. George, 1989. High frequency plant regeneration through somatic embryogenesis in finger millet (Eleusine coracana L.). Gaertn Plant Sci 61: 127–130.

    Google Scholar 

  • Fransz P.F., N.C.A.De Ruijter & H.N. Schel, 1989. Isozymes as biochemical and cytochemical markers in embryogenic callus cultures of maize (Zea mays L.). Plant Cell Rep 8: 67–70.

    Google Scholar 

  • Gamborg O.L., R.A. Miller & K. Ojima, 1968. Nutrient requirements of suspensions cultures of soybean root cells. Exp Cell Res 50: 148–151.

    Google Scholar 

  • Gamborg O.L., F. Constabel & J.P. Shyluk, 1974. Organogenesis in callus from shoot apices of Pisum sativum. Physiol Plant 30: 125–128.

    Google Scholar 

  • Gharyal P.K. & S.C. Maheshwari, 1983. Genetic and physiological influences on differenciation in tissue cultures of legumes, Lathyrus sativus. Theor Appl Genet 66: 123–126.

    Google Scholar 

  • Huyghe C., 1990. Les cultures in vitro chez les légumineuses à grosses graines. Agronomie 10: 29–49.

    Google Scholar 

  • Jacobsen H.J. & W. Kisely, 1984. Induction of somatic embryos in pea, Pisum sativum L.. Plant Cell Tissue Organ Culture 3: 319–324.

    Google Scholar 

  • Kao K.N. & M.R. Mychialuk, 1981. Embryoid formation in alfalfa cell suspension cultures from different plants. In Vitro 17: 645–649.

    Google Scholar 

  • Kartha K.K., O.L. Gamborg & F. Constabel, 1974. Regeneration of pea (Pisum sativum L.) plants from shoot apical meristems. Z. Pflanzenphysiol 72: 172–176.

    Google Scholar 

  • Kisely K., J.R. Myers, P.A. Lazzeri, G.B. Collins & H.J. Jacobsen, 1987. Plant regeneration via somatic embryogenesis in pea. Plant Cell Rep 6: 305–308.

    Google Scholar 

  • Kisely W. & H.J. Jacobsen, 1990. Somatic embryogenesis from pea embryos and shoot apices. Plant Cell Tissue and Organ Culture 20: 7–14.

    Google Scholar 

  • Kumar K.B., N. Pareer, S.K. Pillai & A. Pillai, 1982. Callus development, shoot formation and somatic embryogenesis in Coriandrum sativum L. in vitro. Beitrage Biologische Pflanzen 57: 369–376.

    Google Scholar 

  • Lambert, M., 1991. Embryogenèse somatique chez le soja (Glycine max L. Merrill). Thèse Université Paris Sud. Centre d'Orsay. 179 pp.

  • Lehminger-Mertens R. & H.J. Jacobsen, 1989. Plant regeneration from pea protoplasts via somatic embryogenesis. Plant Cell Rep 8: 379–382.

    Google Scholar 

  • Malberg R.L., 1979. Regeneration of whole plants from callus culture of diverse genetic liens of Pisum sativum L. Planta 146: 243–244.

    Google Scholar 

  • Martins I.S. & M.R. Sondahl, 1984. Early stage of somatic embryo differentiation from callus cells of bean (Phaseolus vulgaris L.) growth in liquid. J Plant Physiol 117: 97–103.

    Google Scholar 

  • McGee E.G., E.G. Williams, G.B. Collins & D.F. Hildebrand, 1989. Somatic embryogenesis in Trifolium: Protein profiles associated with high and low frequency regeneration. J Plant Physiol 135: 306–312.

    Google Scholar 

  • Mronginski L.A. & K.K. Kartha, 1984. Tissue culture of legumes for crop improvement. Plant Breed 2: 215–264.

    Google Scholar 

  • Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.

    Google Scholar 

  • Saâdi, A., 1991. Régénération de plantes de pois (Pisum sativum L.) par embryogenèse somatique. Thèse, Institut National Agronomique, Paris-Grignon. 162 pp.

  • Saxena P.K. & J. King, 1987. Morphogenesis in lentil: Plant regeneration from callus culture of lens culinaris Medik via somatic embryogenesis. Plant Sci Rep 52: 223–227.

    Google Scholar 

  • Weigl R.C. & K.W. Hughes, 1985. Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants. Plant Cell Tissue Organ Culture 5: 151–162.

    Google Scholar 

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  1. M. Bencheikh

    Present address: , BP220, 2000, Chlef, Algeria

Authors and Affiliations

  1. Institut National Agronomique P-G, 16 rue Claude Bernard, 75231, Paris Cedex 05, France

    M. Bencheikh & A. Gallais

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  1. M. Bencheikh
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  2. A. Gallais
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Bencheikh, M., Gallais, A. Study of the variation in the somatic embryogenesis ability of some pea lines (Pisum sativum L. and Pisum arvense L.). Euphytica 90, 251–256 (1996). https://doi.org/10.1007/BF00027473

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  • DOI: https://doi.org/10.1007/BF00027473

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