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In vitro clonal multiplication of mature trees of Dalbergia sissoo Roxb.

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Abstract

Multiple shoots were obtained from nodal explants of 30-year-old trees of Dalbergia sissoo Roxb. on a defined medium, MS (Murashige & Skoog medium) supplemented with auxin-cytokinin combinations. IAA (Indole accetic acid) alone promoted 15% rooted shoot buds. A combination of IAA+Kn (Kinetin) gave 100% rooted shoot buds. A combination of NAA (napthaleneacetic acid) + Kn and NAA+BAP (6-benzylaminopurine) also gave high percentages of rooted shoot buds. Ascorbic acid in the medium prevented the death of callus and plantlets, which followed darkening of the medium.

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Authors and Affiliations

  1. Tissue Culture and Pharmacognosy Lab., Department of Botany, School of Life Science, Visva-Bharati, 731235, Santiniketan, India

    Swapan K. Datta, Karabi Datta & T. Pramanik

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  1. Swapan K. Datta
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  2. Karabi Datta
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  3. T. Pramanik
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Datta, S.K., Datta, K. & Pramanik, T. In vitro clonal multiplication of mature trees of Dalbergia sissoo Roxb.. Plant Cell Tiss Organ Cult 2, 15–20 (1982). https://doi.org/10.1007/BF00033548

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  • DOI: https://doi.org/10.1007/BF00033548

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