Skip to main content
SpringerLink
Log in

Induction, maintenance, and differentiation of rice callus cultures on ammonium as sole nitrogen source

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Rice callus was initiated from cultured anthers and was maintained on medium containing ammonium as sole nitrogen source and supplemented with either a buffer or an organic acid. Plant regeneration also was obtained on this medium, but the morphogenetic capacity of the callus culture declined as rapidly as on a medium containing a mixture of ammonium and nitrate. The results of the studies suggest that organic acids provide an additional function beyond that of buffering the medium during growth on ammonium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Behrend J, Mateles RI (1976). Nitrogen metabolism in plant cell suspension cultures II. Role of organic acids during growth on ammonia. Plant Physiol 58: 510–512

    Google Scholar 

  2. Chaleff RS (1980). Tissue culture in rice improvement: An overview. In Innovative Approaches to Rice Breeding, pp 81–91 Los Banos, Philippines: International Rice Research Institute

    Google Scholar 

  3. Chaleff RS, Stolarz A (1981). Factors influencing the frequency of callus formation among cultured rice (Oryza sativa) anthers. Physiol Plant 51:201–206

    Google Scholar 

  4. Dougall DK, Verma DC (1978). Growth and embryo formation in wild carrot suspension cultures with ammonium ion as a sole nitrogen source. In Vitro 14: 180–182

    Google Scholar 

  5. Dougall DK, Weyrauch KW (1980). Abilities of organic acids to support growth and anthocyanin accumulation by suspension cultures of wild carrot cells using ammonium as the sole nitrogen source. In Vitro 16:969–975

    Google Scholar 

  6. Fukunaga Y, King J, Child JJ (1978). The differential effects to TCA-cycle acids on the growth of plant cells cultured in liquid media containing various nitrogen sources. Planta 139:199–202

    Google Scholar 

  7. Gamborg OL, Shyluk JP (1970). The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol 45:598–600

    Google Scholar 

  8. Linsmaier EM, Skoog F (1965). Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127

    Google Scholar 

  9. Martin SM, Rose D (1976). Growth of plant cell (Ipomoea) suspension cultures at controlled pH levels. Can J Bot 54:1264–1270

    Google Scholar 

  10. Martin SM, Rose D, Hui V (1977). Growth of plant cell suspension cultures with ammonium as the sole source of nitrogen. Can J Bot 55:2838–2843

    Google Scholar 

  11. Snedecor GW, Cochran WG (1967). Statistical Methods. Ames, Iowa: Iowa State Univ. Press

    Google Scholar 

  12. Tazawa M, Reinert J (1969). Extracellular and intracellular chemical environments in relation to embryogenesis in vitro. Protoplasma 68:157–173

    Google Scholar 

  13. Wetherell DF, Dougall DK (1976). Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Plant Physiol 37:97–103

    Google Scholar 

  14. Yatazawa M, Furuhashi K (1968). Nitrogen sources for the growth of rice callus tissue. Soil Sci Plant Nutr 14:73–79

    Google Scholar 

  15. Yoshida S, Forno D, Cock JH, Gomez KA (1972). Routine procedure for growing rice plants in culture solution. In Laboratory Manual for Physiological Studies of Rice. Los Banos, Philippines: The International Rice Research Institute, pp 53–57

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Central Research and Development, Experimental Station, E.I. du Pont de Nemours & Company, 19801, Wilmington, DE, USA

    R S Chaleff

Authors
  1. R S Chaleff
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chaleff, R.S. Induction, maintenance, and differentiation of rice callus cultures on ammonium as sole nitrogen source. Plant Cell Tiss Organ Cult 2, 29–37 (1982). https://doi.org/10.1007/BF00033550

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00033550

Keywords

Search

Navigation