Abstract
Production of encapsulatable unit, scale-up of the production system, and encapsulation technologies were developed as an integrated technology for transplant production using somatic embryos of celery and carrot. Encapsulatable units, somatic embryos improved in their quality for encapsulation, showed 80% conversion without encapsulation and any nutrient supply on a nursery medium in a greenhouse. The high conversion ability was also shown by carrot encapsulatable unit produced with a newly developed 8-liter culture jar, where 5.09×105 torpedo stage embryos were produced and 4.10×104 encapsulatable units were obtained from them.
A novel self-breaking gel beads and sustained release microcapsule as an artificial endosperm were developed for the encapsulation of carrot embryos. Also a whole production system for synthetic seed was developed with which 80 thousand beads could be produced per day. The encapsulated carrot embryos showed 52% conversion frequency after sowing on a humid soil in greenhouse.
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Abbreviations
- BA:
-
benzyladenine
- GA:
-
gibberellic acid
- MC:
-
microcapsule
References
Fujii JA, Slade D & Redenbaugh K (1989) Maturation and greenhouse planting of alfalfa artificail seed. In Vitro Cell. Dev. Biol. 25: 1179–1182
Fujii JA, Slade D, Aguirre-Rascon J & Redenbaugh K (1992) Field planting of alfalfa artificial seeds. In Vitro Cell Dev. Biol. 28: 73–80
Garret RE, Smith NE, Mehlschau JJ & Redenbaugh MK (1991) Gel encapsulation of tomato seeds, Appl. Eng. Agric., 7(1): 25–31
Gray D (1987) Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration. HortScience 22: 810–814
Janick J, Kitto S & Kim Y-H (1989) Production of synthetic seed by desiccation and encapsulation. In Vitro Cell. Dev. Biol. 25: 1167–1172
Janick J, Kim Y-H, Kitto S & Saranga Y (1993) Desiccated synthetic seed. In: Redenbaugh K (Ed) Synseeds (pp 11–33). CRC Press, Boca Raton
Kouno Y (1989) Method of applying gel coating to plant seeds United States Patent 4,808,430
McKersie B, Senaratna T, Bowley S, Brown D, Krochko J & Bewley J (1989) Application of artificial seed technology in the production of hybrid alfalfa (Medicago sativa L.). In Vitro Cell. Dev. Biol. 25: 1183–1188
Molle F, Duquis J-M, Ducos J-P, Anselm A, Crolus-Savidan I, Petiard V & Freyssinet G (1993) Carrot somatic embryogenesis and its application to synthetic seeds. In: Redenbaugh K (Ed) Synseeds (pp 257–287). CRC Press, Boca Raton
Onishi N, Mashiko T & Okamoto A (1992) Cultural system producing encapsulatable units of synthetic seeds in celery. Acta Hortic. 319: 113–118
Redenbaugh K, Slade D, Viss P & Fujii J A (1987) Encapsulation of somatic embryos in synthetic seed coats HortScience 22(5): 803–809
Redenbaugh K, Fujii JA & Slade D (1991) Synthetic seed technology. In: Vasil IK (Ed) Scale-up and Automation in Plant Propagation (pp 35–74). Academic Press, San Diego
Redenbaugh K, Fujii J A & Slade D (1993) Hydrated Coating for Synthetic Seeds. In: Redenbaugh K (Ed) Synseeds (pp 35–46). CRC Press, Boca Raton
Roberts DR, Webster FB, Flinn BS, Lazaroff WR & Cyr DR (1992) Somatic emryogenesis of spruce. In: Redenbaugh K (Ed) Synseeds (pp 427–450). CRC Press, Boca Raton
Sakamoto Y, Umeda S & Ogishima H (1991) Artificial seed comprising a sustained-release granule. United States Patent 5,010,865
Sakamoto Y, Mashiko T, Suzuki A, Kawata H & Iwasaki A (1992) Development of encapsulation technology for synthetic seeds Acta Hortic. 319: 71–76
Sakamoto Y, Onishi N & Hirosawa T (1994) Delivery system for tissue culture by encapsulation. In: Aitken-Christie J et al. (Eds) Automation and Environmental Control in Plant Tissue Culture, Kluwer Academic Publishers, Dordrecht, Netherlands (in press)
Sanada M, Sakamoto Y, Hayashi M, Mashiko T, Okamoto A & Onishi N (1993) Celery and lettuce. In: Redenbaugh K (Ed) Synseeds (pp 305–327). CRC Press, Boca Raton
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Onishi, N., Sakamoto, Y. & Hirosawa, T. Synthetic seeds as an application of mass production of somatic embryos. Plant Cell Tiss Organ Cult 39, 137–145 (1994). https://doi.org/10.1007/BF00033921
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DOI: https://doi.org/10.1007/BF00033921