Skip to main content
Log in

Growth and morphogenesis of immature embryos of sweet potato (Ipomoea batatas L.) in vitro

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Sweet potato (Ipomoea batatas L.) embryos excised from the fertilized ovules of 6- to 12-day-old capsules were cultured on MS medium supplemented with NAA, BA, GA separately and in combinations. GA was found essential for initial morphogenesis of globular and heart stages. Seedlings were recovered from late globular stage onwards but recovery was best from advanced embryo stages. Differentiated embryos produced multiple shoots on MS medium +1μM NAA÷2μM BA +0.5μM GA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Similar content being viewed by others

References

  • Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158

    Google Scholar 

  • Henderson JHM, Phills BR & Whatley BT (1984) Sweet potato. In: Sharp WR, Evans DA, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 2 (pp 302–326). Macmillan Publishing Co. New York

    Google Scholar 

  • Martin FW (1982) Analysis of the incompatibility and sterility of sweet potato. In: Villareal RL & Griggs TD (Eds) Proc. Int. Sump. Sweet Potato (pp 275–283). AVRDC. Taiwan, China

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Raghavan V (1966) Nutrition, growth and morphogenesis of plant embryos. Biol. Rev. 41: 1–58

    Google Scholar 

  • Vijayabai K (1976) Cytogenetical studies in the genus Ipomoea. In: Central Tuber Crops Research Institute Annual Report (pp 82–83). CTCRI, Trivandrum, India

    Google Scholar 

  • Vijayabai K (1977) Cytogenetical studies in the genus Ipomoea. In: Central Tuber Crops Research Institute Annual Report (pp 98–100). CTCRI, Trivandrum, India

    Google Scholar 

  • Wedderburn MM (1967) A study of hybridization involving the sweet potato and related species. Euphytica 16: 69–75

    Google Scholar 

  • White PR (1963) The Cultivation of Animal and Plant Cells. Ronald Press, New York

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mukherjee, A., Unnikrishnan, M. & Nair, N.G. Growth and morphogenesis of immature embryos of sweet potato (Ipomoea batatas L.) in vitro. Plant Cell Tiss Organ Cult 26, 97–99 (1991). https://doi.org/10.1007/BF00036112

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00036112

Key words

Navigation