Abstract
Sweet potato (Ipomoea batatas L.) embryos excised from the fertilized ovules of 6- to 12-day-old capsules were cultured on MS medium supplemented with NAA, BA, GA separately and in combinations. GA was found essential for initial morphogenesis of globular and heart stages. Seedlings were recovered from late globular stage onwards but recovery was best from advanced embryo stages. Differentiated embryos produced multiple shoots on MS medium +1μM NAA÷2μM BA +0.5μM GA.
Access this article
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Similar content being viewed by others
References
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158
Henderson JHM, Phills BR & Whatley BT (1984) Sweet potato. In: Sharp WR, Evans DA, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 2 (pp 302–326). Macmillan Publishing Co. New York
Martin FW (1982) Analysis of the incompatibility and sterility of sweet potato. In: Villareal RL & Griggs TD (Eds) Proc. Int. Sump. Sweet Potato (pp 275–283). AVRDC. Taiwan, China
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Raghavan V (1966) Nutrition, growth and morphogenesis of plant embryos. Biol. Rev. 41: 1–58
Vijayabai K (1976) Cytogenetical studies in the genus Ipomoea. In: Central Tuber Crops Research Institute Annual Report (pp 82–83). CTCRI, Trivandrum, India
Vijayabai K (1977) Cytogenetical studies in the genus Ipomoea. In: Central Tuber Crops Research Institute Annual Report (pp 98–100). CTCRI, Trivandrum, India
Wedderburn MM (1967) A study of hybridization involving the sweet potato and related species. Euphytica 16: 69–75
White PR (1963) The Cultivation of Animal and Plant Cells. Ronald Press, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mukherjee, A., Unnikrishnan, M. & Nair, N.G. Growth and morphogenesis of immature embryos of sweet potato (Ipomoea batatas L.) in vitro. Plant Cell Tiss Organ Cult 26, 97–99 (1991). https://doi.org/10.1007/BF00036112
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00036112