Abstract
Physiological concentrations of ethylene in the growth medium of autotropic suspension culture Chenopodium rubrum L. cells reduced the activity of cell wall bound invertase by 25 – 47%, compared to controls. Northern blot analysis using homologous probe binding to total RNA preparations revealed that reduction in specific activity was paralleled by repression of the corresponding gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abeles FB, Morgan PW and Saltveit ME (1992) Ethylene in Plant Biology, 2nd ed. San Diego: Academic Press, Inc., pp 245–251
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72: 248–254
Buddendorf-Joosten JMC and Woltering EJ (1994) Components of the gaseous environment and their effects on plant growth and development in vitro. Plant Growth Regulation 15: 1–16
Eschrich W (1980) Free space invertase, its possible role in phloem unloading. Ber Deutsch Bot Ges 93: 363–378
Godt D, Riegel A and Roitsch T (1995) Regulation of sucrose synthase expression in Chenopodium rubrum: Characterization of sugar induced expression in photoautotrophic suspension cultures and sink tissue specific expression in plants. J Plant Physiol 246: 231–238
Hubbard NL, Huber SC and Pharr DM (1989) Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melon L.) fruits. Plant Physiol 91: 1527–1534
Hüsemann W and Barz W (1977) Photoautotrophic growth and photosynthesis in cell suspension cultures of Chenopodium rubrum. Physiol Plant 40: 77–81
Iki K, Sekiguchi K, Kurata K, Tada T, Nakagawa H, Ogura N and Takehana H (1978) Immunological properties of β-fructofuranosidase from ripening tomato fruit. Phytochem 17: 311–312
Konings A and Jackson MB (1979) A relationship between rates of ethylene production by roots and the promoting or inhibiting effects of exogenous ethylene and water on root elongation. Z Pflanzenphysiol 92: 385–397
Lingle SE and Dunlap JR (1991) Sucrose metabolism and IAA and ethylene production in muskmelon ovaries. J Plant Growth Regul 10: 167–171
Miller EM and Chourey PS (1992) The maize invertase deficient miniature-1 seed mutation is associated with aberrant pedical and endosperm development. Plant Cell 4: 297–305
Roitsch T, Bittner M and Godt D (1995) Induction of apoplastic invertase of Chenopodium rubrum by D-glucose and a glucose analogue and tissue specific expression suggest a role in sink source regulation. Plant Physiol 108: 285–294
Roitsch T and Tanner W (1994) Expression of a sugar-transporter gene family in a photoautotrophic suspension culture of Chenopodium rubrum L. Planta 193: 365–371
Sanders IO, Harpham NVJ, Raskin I, Smith AR and Hall MA (1991) Ethylene binding in wildtype and mutant Arabidopsis thaliana (L.) Heyth. Annals of Botany 68: 97–103
Seifert F, Langebartels C, Boller T and Grossmann K (1994) Ethylene and 1-ACC involved in induction of chitinase and β-1,3-glucanase activity in sunflower cell-suspension. Planta 192: 431–440
Sisler E (1991) Ethylene binding components in plants. In: Mattoo A and Suttle J (eds) The Plant Hormone Ethylene, pp 81–99. Boca Raton, FL: CRC Press
Ullmann J, Prikryl Z, Machackova I and Krekule J (1986) Ethylene production in seedlings of Chenopodium rubrum, as influenced by seedlings age, light, and CO2 concentration. Biochem Physiol Pflanzen 181: 391–396
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Linden, J.C., Ehneß, R. & Roitsch, T. Ethylene regulation of apoplastic invertase expression in autotrophic cells of Chenopodium rubrum . Plant Growth Regul 19, 219–222 (1996). https://doi.org/10.1007/BF00037794
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00037794