Abstract
Plant regeneration through direct somatic embryogenesis of leaf blade explants from in vitro propagated plants of Agave victoria-reginae Moore, is described. Somatic embryogenesis was evident in a 6-week period on agarsolidified MS medium supplemented with L2 vitamins and 2,4-dichlorophenoxyacetic acid (1,4 µM), and germination of somatic embryos was achieved after 8 weeks on half-strength MS medium and 4 weeks on half-strength SH medium, both lacking growth regulators. Hyperhydricity of somatic embryos and plantlets was reduced by the use of vented culture vessel lids during the last 4 weeks on SH medium. Shoot proliferation was obtained, and hyperhydricity was eliminated on a modified MS medium (with NH4NO3 reduced to 5 mN) supplemented with kinetin (4.6 µM) and 1-naphthaleneacetic acid (1.6 µM) and the use of vented culture vessel lids.
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Abbreviations
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- MS:
-
Murashige and Skoog
- LOG-1:
-
MS modified medium by Castro-Concha et al. (1990)
- L2:
-
Phillips and Collins (1979) vitamins
- SH:
-
Schenk and Hildebrandt
References
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Rodríguez-Garay, B., Gutiérrez-Mora, A. & Acosta-Duefias, B. Somatic embryogenesis of Agave victoria-reginae Moore. Plant Cell Tiss Organ Cult 46, 85–87 (1996). https://doi.org/10.1007/BF00039700
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DOI: https://doi.org/10.1007/BF00039700