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Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr.)

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Abstract

Somatic embryogenesis was achieved in protoplast cultures of coffee. Protoplasts were isolated from cell suspension-derived somatic embryos of Coffea canephora. After repeated subculture in a medium supplemented with 0.5 mg/l of each of kinetin, 2,4-dichlorophenoxyacetic acid (2,4-D), and naphthaleneacetic acid (NAA), microcalli developed. Transfer of these microcalli to a medium lacking growth regulators resulted in the formation of globular embryos. Upon subculture without growth regulators they grew to well-differentiated embryos, Eventually some of them developed to plantlets which were transferred to the greenhouse for further observation.

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Authors and Affiliations

  1. Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, D-6000, Frankfurt am Main 1, FRG

    Christian Schöpke & Hans-Willy Kohlenbach

  2. Laboratorio de Cultivos de Tejidos, Centro Agronómico Tropical de Investigación y Enseñanza, 7170, Turrialba, Costa Rica

    Ludwig E. Müller

Authors
  1. Christian Schöpke
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  2. Ludwig E. Müller
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  3. Hans-Willy Kohlenbach
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Schöpke, C., Müller, L.E. & Kohlenbach, HW. Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee (Coffea canephora P. ex Fr.). Plant Cell Tiss Organ Cult 8, 243–248 (1987). https://doi.org/10.1007/BF00040951

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  • DOI: https://doi.org/10.1007/BF00040951

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