Abstract
Stem explants obtained from a mature tree of Ziziphus mauritiana Lamk were grown on modified Murashige and Skoog medium containing 3800 mg l-1 potassium nitrate, 2475 mg l-1 ammonium nitrate, 11 μM benzyladenine and 0.5 μM indole-3-acetic acid. During successive subcultures 15–20 shoots per inoculum were produced. Rooting was induced by pretreatment with 50 μM indolebutyric acid or 1-naphthaleneacetic acid for 24 h followed by transfer to auxin-free White's medium. Plantlets grew well in a soil and vermiculite mixture.
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Abbreviations
- IAA:
-
Indole-3-acetic acid
- NAA:
-
1-naphthaleneacetic acid
- BA:
-
benzyladenine
- MS:
-
Murashige and Skoog
References
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Mathur, N., Ramawat, K.G. & Nandwani, D. Rapid in vitro multiplication of jujube through mature stem explants. Plant Cell Tiss Organ Cult 43, 75–77 (1995). https://doi.org/10.1007/BF00042675
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DOI: https://doi.org/10.1007/BF00042675