Abstract
Using low concentrations of picloram (0.06 mg/l), embryoids were formed on the surface of leaf-derived callus of pea, Pisum sativum L. (c.v. Dippes Gelbe Victoria) upon transfer to liquid medium. After some days in culture, embryoids spontaneously separated from the calli, and developed into torpedo-shaped embryos, which were transferred to solid medium. In a second series of experiments, embryos were also formed by mutant 489C and a genetic line of Pisum arvense, which additionally exhibited embryogenesis also from epicotyl-derived callus. Some of the embryos showed root formation, but no shoot morphogenesis occurred. In a limited number of cases, an additional root was formed in the apparent shoot apical region after 2–5 days.
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References
Beversdorf WD, Bingham ET (1977) Degrees of differentiation obtained in tissue cultures of Glycine species. Crop Sci. 17:307–311
Beyl CA, Sharma GC (1983) Picloram induced somatic embryogenesis in Gasteria and Haworthia. Plant Cell, Tissue & Organ Culture 2:123–132
Christianson ML, Warnick DA, Carlson PS (1983) A morphogenetic competent soybean suspension culture. Science Vol. 222, No. 4624:632–634
Crocomo OJ, Sharp WR, Peters JE (1976) Plantlet morphogenesis and the control of callus growth and root induction of Phaseolus vulgaris with the addition of a bean seed extract. Z. Pflanzenphysiol. 78:456–460
Collins GB, vian WE, Phillips GC (1978) Use of 4-amino-3,4,6-trichloro picolinic acid as an auxin source in plant tissue culture. Crop Sci 18: 286–288
Jacobsen HJ, Ingensiep HW, Herlt M, Kaul MLH (1980) Tissue culture studies in Pisum sativum L. in: Plant Cell Cultures: Results and perspectives F.Sala, B.Parisi, R.Cella, O.Ciferri (eds.) Elsevier/North Holland, Amsterdam, p 319–324
Malmberg RL (1979) Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L. Planta 146: 243–244
Mroginski LA, Kartha KK (1981) Regeneration of pea (Pisum sativum L. cv. Century) plants by in vitro culture of immature leaflets. Plant Cell Reports 1:64–66
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473–497
Phillips GC, Collins GB (1981) Induction and development of somatic embryos from cell suspension cultures of soybean. Plant Cell, Tissue & Organ Culture 1: 123–129
Sinha RR, Das K, Sen SK (1983) Plant regeneration from stem derived callus of the seed legume Lathyrus sativum L. Plant Cell, Tissue & Organ Culture 2:67–76
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Jacobsen, HJ., Kysely, W. Induction of somatic embryos in pea, Pisum sativum L. . Plant Cell Tiss Organ Cult 3, 319–324 (1984). https://doi.org/10.1007/BF00043083
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DOI: https://doi.org/10.1007/BF00043083