Abstract
Cleistanthus collinus Benth. was micropropagated using nodal explants on MS medium supplemented with 2.2 μM benzyladenine (BA). April to June was the best time for initiating shoot cultures. Shoot proliferation was enhanced when the BA concentration was lowered to 1.1 μM. Rooting was achieved on half-strenth MS medium with 22.8 μM indole-3-acetic acid for 7 days and continuous darkness for the first 72 h of the 7 days.
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Abbreviations
- MS:
-
Murashige & Skoog's medium
- WPM:
-
Woody Plant Medium
- BA:
-
6-benzyladenine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- NAA:
-
1-naphthalenacetic acid
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Quraishi, A., Koche, V. & Mishra, S.K. In vitro micropropagation from nodal segments of Cleistanthus collinus . Plant Cell Tiss Organ Cult 45, 87–91 (1996). https://doi.org/10.1007/BF00043433
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DOI: https://doi.org/10.1007/BF00043433