Abstract
Rhododendron simsii ‘Hellmut Vogel’ was regenerated using different types of explants, auxins and cytokinins. After a callus induction phase, with 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid, adventitious shoot regeneration was obtained on a medium supplemented with thidiazuron or zeatin. With thidiazuron shoots were small and a subsequent elongation step was required before rooting. An elongation step was not required when zeatin was used. The duration of the callus induction phase was negatively correlated with the regeneration capacity.
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Abbreviations
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- 2iP:
-
6-(γ-γ-dimethylallylamino)purine
- NOA:
-
ß-naphthoxyacetic acid
- BA:
-
6-benzyladenine
- IBA:
-
1-H-indole-3-butyric acid
- IAA:
-
1-H-indole-3-acetic acid
- TDZ:
-
thidiazuron
- WPM:
-
woody plant medium
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Mertens, M., Werbrouck, S., Samyn, G. et al. In vitro regeneration of evergreen azalea from leaves. Plant Cell Tiss Organ Cult 45, 231–236 (1996). https://doi.org/10.1007/BF00043635
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DOI: https://doi.org/10.1007/BF00043635