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Influence of media and growth regulators on somatic embryogenesis and plant regeneration for production of primary triticales

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Abstract

Basal media and plant growth regulators were tested for the promotion of somatic embryogenesis from immature wheat-rye hybrid embryos. Influence of growth regulators and chilling on plant regeneration were tested on two media. A medium containing four amino acids-glutamine, arginine, glycine and aspartic acid-as the nitrogen source, promoted the production of, on average, twice as much embryogenic callus as the other media, and somatic embryos developed well. The growth regulator dicamba was significantly better than 2,4-dichlorophenoxyacetic acid in promoting somatic embryogenesis and subsequent plant regeneration. Germination of somatic embryos on both regeneration media was enhanced by cold treatment. Supplementing 190-2 plant regeneration medium with a combination of α-naphthaleneacetic acid + benzyladenine, indole-3-acetic acid + kinetin or indole-3-acetic acid + zeatin resulted in equally high germination rates.

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Abbreviations

190-2:

Plant regeneration medium of Chuang & Jia

2,4-d :

2,4d Dichlorophenoxyacetic acid

Dicamba:

3,6-Dichloro-o-anisic acid

AA:

Amino acid medium of Müller & Grafe

IAA:

Indole-3-acetic acid

BA:

Benzyladenine

NAA:

α-Naphthaleneacetic acid

References

  • Bhaskaran S & Smith RH (1990) Regeneration in cereal tissue culture: A review. Crop Sci. 30: 1328–1336

    Google Scholar 

  • Carman JG (1988) Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments. Planta 175: 417–424

    Google Scholar 

  • Carman JG, Jefferson NE & Campbell WF (1988a) Induction of embryogenicTriticum aestivum L. calli. I. Quantification of genotype and culture medium effects. Plant Cell Tiss. Org. Cult. 12: 83–95

    Google Scholar 

  • Carman JG, Jefferson NE & Campbell WF (1988b) Induction of embryogenicTriticum aestivum L. calli. II. Quantification of organic addenda and other culture variable effects. Plant Cell Tiss. Org. Cult. 12: 97–110

    Google Scholar 

  • Chang FY & Vanden Born WH (1971) Dicamba uptake, translocation, metabolism and selectivity. Weed Sci. 19: 113–117

    Google Scholar 

  • Chu CC, Wang CC & Sun CS (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen source. Sci. Sin. 28: 659–668

    Google Scholar 

  • Galiba G & Yamada Y (1988) A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation. Plant Cell Rep. 7: 55–58

    Google Scholar 

  • Gamborg OL (1970) The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 45: 372–375

    Google Scholar 

  • Grimes HD & Hodges TK (1990) The inorganic NO3:NH4 ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of indica rice (Oryza sativa L.). J. Plant Physiol. 136: 362–367

    Google Scholar 

  • He DG, Yang YM & Scott KJ (1988) A comparison of scutellum callus and epiblast callus induction in wheat: The effect of genotype, embryo age and medium. Plant. Sci. 57: 225–233

    Google Scholar 

  • Henry Y & De Buyser J (1981) Float cultures of wheat anthers. Theor. Appl. Genet. 60: 77–79

    Google Scholar 

  • Husinger H & Schauz K (1987) The influence of dicamba on somatic embryogenesis and frequency of plant regeneration from cultured immature embryos of wheat (Triticum aestivum L.) Plant Breed. 98: 119–123

    Google Scholar 

  • Immonen AST (1993a) Amino acid medium for somatic embryogenesis from immature triticale (XTriticosecale Wittmack) embryos. Cer. Res. Comm. 21: 51–55

    Google Scholar 

  • Immonen AST (1993b) Comparison of callus culture with embryo culture at different times of embryo rescue for primary triticale production. Euphytica 70: 185–190

    Google Scholar 

  • Immonen AST, Varughese G, Pfeiffer WH & Mujeeb-Kazi A (1993) Crossability of tetraploid and hexaploid wheats with ryes for primary triticale production. Euphytica 65: 203–210

    Google Scholar 

  • INSTAT (1990) PC Teaching Guide, Statistical Services Centre. University of Reading, U.K.

    Google Scholar 

  • Jenes B & Pauk J (1989) Plant regeneration from protoplast derived calli in rice (Oryza sativa L.) using dicamba. Plant Sci. 63: 187–198

    Google Scholar 

  • Kaltsikes PJ & Gustafson JP (1986) Triticale (Triticosecale): Production through embryo culture. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry Vol 2 Crops I (pp 523–529). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  • Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybeans-Nicotiana glauca. Mol. Gen. Genet. 150: 225–230

    Google Scholar 

  • Mujeeb-Kazi A & Kimber G (1985) The production, cytology and practicability of wide crosses in the Triticeae. Cer. Res. Comm. 13: 111–124

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Muyuan Z, Abing X, Miaobao Y, Chunnong H, Zhilong Y, Linji W & Jianjun Y (1990) Effects of amino acids on callus differentiation in barley anther culture. Plant Cell Tiss. Org. Cult. 22: 201–204

    Google Scholar 

  • Nakamura C & Keller WA (1982) Callus proliferation and plant regeneration from immature embryos of hexaploid triticale. Z. Pflanzenzüchtg 88: 137–160

    Google Scholar 

  • Olsen FL (1987) Induction of microspore embryogenesis in cultured anthers ofHordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res. Comm. 52: 393–404

    Google Scholar 

  • Ozias-Akins P & Vasil IK (1983) Improved efficiency and normalization of somatic embryogenesis inTriticum aesticum (wheat). Protoplasma 117: 40–44

    Google Scholar 

  • Padgett PE & Leonard RT (1994) An amino-acid-grown maize cell line for use in investigating nitrate assimilation. Plant Cell Rep. 13: 504–509

    Google Scholar 

  • Papenfuss JM & Carman JG (1987) Enhanced regeneration from wheat callus cultures using dicamba and kinetin. Crop Sci. 27: 588–593

    Google Scholar 

  • Rajasekaran K, Vine J & Mullins MG (1982) Dormancy in somatic embryos and seeds ofVitis: changes in endogenous abscisic acid during embryogeny and germination. Planta 154: 139–144

    Google Scholar 

  • SAS User's Guide (1985) Version 5 edition, Cary, N.C., USA, SAS Institute

  • Shao ZZ & Taira T (1990) Production of primary triticale from calluses of immature abnormal hybrid embryos betweenTriticum durum andSecale cereale. Plant Breed. 105: 81–88

    Google Scholar 

  • Shetty K & Asano Y (1991) The influence of organic nitrogen sources on the induction of embryogenic callus inAgrostis alba L. J. Plant Physiol. 139: 82–85

    Google Scholar 

  • Snedecor GW & Cochran WG (1967) Statistical methods. 6th edition, The lowa State University Press, Ames, Iowa, USA

    Google Scholar 

  • Skokut TA, Manchester J & Schaefer J (1985) Regeneration in alfalfa tissue culture. Plant Physiol. 79: 579–583

    Google Scholar 

  • Thompson JA, Abdullah R & Cocking EC (1986) Protoplast culture of rice (Oryza sativa L.) using media solidifed with agarose. Plant Sci. 47: 123–133

    Google Scholar 

  • Wang X & Hu H (1984) The effect of potato II medium for triticale anther culture. Plant Sci. Lett. 36: 237–239

    Google Scholar 

  • Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J. Plant Physiol. 128: 193–218

    Google Scholar 

  • Varughese G, Barker T & Saari E (1987) Triticale. CIMMYT, Mexico, D.F. (32 p)

    Google Scholar 

  • Zimmy J & Lörz H (1989) High frequency of somatic embryogenesis and plant regeneration of rye (Secale cereale L.). Plant Breeding 102: 89–100

    Google Scholar 

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Author notes

  1. A. S. T. Immonen

    Present address: Institute of Crop and Soil Science, Plant Breeding Section, Agricultural Research Centre, FIN-31600, Jokioinen, Finland

Authors and Affiliations

  1. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, D.F., Mexico

    A. S. T. Immonen

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Immonen, A.S.T. Influence of media and growth regulators on somatic embryogenesis and plant regeneration for production of primary triticales. Plant Cell Tiss Organ Cult 44, 45–52 (1996). https://doi.org/10.1007/BF00045912

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