Skip to main content
SpringerLink
Log in

High frequency somatic embryogenesis in Coffea canephora

Induction conditions and histological evolution

  • Orginal Research Papers
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Leaf explants of Coffea canephora (P. ex Fr.) produced a friable yellow callus when they were cultured on a conditioning basal medium with 2.2 μM 2,4-D, 2.4 μM IBA and 9.8 μM 2iP for 4 weeks then on an induction basal medium with 4.4 μM 2,4-D and 17.8 μM BA for 10 weeks. This calus could be maintained by means of regular subcultures or it could give rise to somatic embryos depending on the culture medium. Cytological studies documented somatic embryogenesis and embryo development.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

2,4-D :

2,4-dichlorophenoxyacetic acid

IBA:

indole-3-butyric acid

2iP:

2-isopentenyladenine

MS:

Murashige & Skoog medium

NAA:

α-naphthaleneacetic acid

NPR:

nucleoplasmic ratio

PGR:

plant growth regulator

References

  • Bieysse D, Gofflot A & Michaux-Ferrière N (1993) Effect of experimental conditions and genotypic variability on somatic embryogenesis in Coffea arabica L. Can. J. of Bot. 71: 1496–1502

    Google Scholar 

  • Dublin P (1984) Techniques de reproduction végétative in vitro et amélioration génétique chez les caféiers cultivés. Café, Cacao, Thé XXXVIII: 231–244

    Google Scholar 

  • Fisher DB (1968) Protein staining of ribboned epon sections for light microscopy. Histochemie 19: 92–96

    Google Scholar 

  • Fitch M (1993) High frequency somatic embryogenesis and plant regeneration from papaya hypocotyl callus. Plant Cell Tiss. Org. Cult. 32: 205–212

    Google Scholar 

  • Lanaud C (1981) Production de plantules de Coffea canephora par embryogenèse somatique réalisée partir de culture in vitro d'ovules. Café, Cacao, Thé (Paris) XXV(4): 231–236

    Google Scholar 

  • Martoja R & Martoja M (1967) Initiation aux techniques de I'histologie animale. Masson et Cie, Paris

    Google Scholar 

  • Michaux-Ferrière N & Dublin P (1987) Embryogenèse somatique chez Coffea arabica. Induction et développement des cellules embryogènes. 12ème Colloque Scientifque International sur le Café, Montreux, ASIC (Paris): 418–425

  • Michaux-Ferrière N, Dublin P & Schwendiman J (1987) Etude histologique de l'embryogenèse somatique à partir d'explants foliaire de Coffea arabica L. Café Cacao, Thé XXXI (2): 103–114

    Google Scholar 

  • Michaux-Ferrière N, Bieysse D, Alvard D & Dublin P (1989) Etude histologique de l'embryogenèse somatique chez Coffea arabica, induite par culture sur milieux uniques de fragments foliaires de génotypes différents. Café, Cacao, Thé XXXIII(4): 207–217

    Google Scholar 

  • Muralidharan EM, Gupta PK & Mascarenhas AF (1989) Plantlet production through high frequency somatic embryogenesis in long term cultures of Eucalyptus citriodora. Plant Cell Rep. 8: 41–43

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nakamura T, Taniguchi T & Maeda E (1992) Studies on somatic embryogenesis of Coffee by scanning electron microscope. Japan. J. Crop Sci. 61: 476–486

    Google Scholar 

  • Nassuth A, Wormer TM, Bouman F & Staritsky G (1980) The histogenesis of callus in Coffea canephora stem explants and the discovery of early embryoid initiation; Acta Bot. Neerl. 29(1): 49–54

    Google Scholar 

  • Pierson ES, Van Lammeren AAM, Schel JHN & Staritsky G (1983) In vitro development of embryoids from punched leaf discs of Coffea arabica. Protoplasma 115: 208–216

    Google Scholar 

  • Schöpke C, Muller L & Kohlenbach HW (1987) Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of Coffee (Coffea canephora P.ex. Fr.). Plant Cell Tiss. Org. Cult. 8: 243–248

    Google Scholar 

  • Söndhal MR & Sharp WR (1977) High frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. 81: 395–408

    Google Scholar 

  • Söndhal MR & Sharp WR (1979) Research in Coffea spp. and applications of tissue culture method in: Sharp, Larsen Paddock & Raghavan (eds) Plant Cell and Tissue Culture. Principles and Applications. (pp 527–584). Ohio State Univ. Press, Columbus, USA

    Google Scholar 

  • Söndhal MR, Salisbury JL & Sharp WR (1979a) SEM characterisation of embryogenic tissue and globular embryos during high frequency somatic embryogenesis in coffee callus cells. Z. Pflanzenphysiol. 94: 185–188

    Google Scholar 

  • Söndhal MR, Spahlinger A & Sharp WR (1979b) A histological study of high frequency and low frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. 94: 101–108

    Google Scholar 

  • Staritsky G (1970) Embryoid formation in callus tissues of coffee. Acta Bot. Neerl. 18: 509–514

    Google Scholar 

  • Vasil V & Vasil K (1986) Plant regeneration from friable embryogenic callus and cell suspension cultures of Zea maysL. J. Plant Physiol. 124: 399–408

    Google Scholar 

  • Yasuda T, Fujii Y & Yamaguchi T (1985) Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant Cell Physiol 26(3): 595–597

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CIRAD-CP et CIRAD, BP 5035, 34032, Montpellier Cédex 1, France

    M. Berthouly & N. M. Michaux-Ferriere

Authors
  1. M. Berthouly
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. M. Michaux-Ferriere
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berthouly, M., Michaux-Ferriere, N.M. High frequency somatic embryogenesis in Coffea canephora . Plant Cell Tiss Organ Cult 44, 169–176 (1996). https://doi.org/10.1007/BF00048196

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00048196

Key words

Search

Navigation