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Plant regeneration from somatic tissue of Rhododendron laetum x aurigeranum

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Abstract

The influence of the source of plant material (greenhouse-grown plants or in vitro shoot cultures), the type of tissue explant (shoot-tip, single-node stem segment, whole leaf, leaf strip or half-leaf section) and growth regulator concentration on shoot regeneration from somatic tissue of Rhododendron laetum × aurigeranum was evaluated. No regeneration response was obtained on explants from greenhouse-grown plants. Adventitious shoots were obtained from callus produced at the basal end of shoot-tip and single-node stem segment explants derived from in vitro-grown shoots cultured on Anderson's medium supplemented with 22.8 μM IAA and 73.8 μM 2iP. The greatest percentage of adventitious shoot regeneration (77%) was induced on leaf sections cultured in the presence of 22.8 μM IAA and 147.6 μM 2iP. Plant regeneration was accomplished with minimal callus formation. This technique represents a further step toward gene manipulation of Rhododendron.

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Abbreviations

IAA:

1-H-Indole-3-acetic acid

2iP:

N-(3-methyl-2-Butenyl)-1H-purin-6 amine

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Author notes

  1. Giovanni Iapichino

    Present address: Instituto di Orticoltura e Floricoltura, Universita' di Palermo, 90100, Italy

Authors and Affiliations

  1. Department of Horticulture, Oregon State University, 97331-2911, Corvallis, OR, U.S.A.

    Giovanni Iapichino, Tony H. H. Chen & Leslie H. Fuchigami

Authors
  1. Giovanni Iapichino
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  2. Tony H. H. Chen
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  3. Leslie H. Fuchigami
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Iapichino, G., Chen, T.H.H. & Fuchigami, L.H. Plant regeneration from somatic tissue of Rhododendron laetum x aurigeranum . Plant Cell Tiss Organ Cult 27, 37–43 (1991). https://doi.org/10.1007/BF00048204

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  • DOI: https://doi.org/10.1007/BF00048204

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