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In vitro establishment and multiplication of Nymphaea hybrid ‘James Brydon’

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Abstract

Rhizome tips were the most suitable explants for in vitro plant regeneration and multiplication of Nymphaea hybrid ‘James Brydon’ on Murashige and Skoog medium containing different concentrations and combinations of indole-3-acetic acid, 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid, 6-benzyladenine (BA), kinetin, 2-isopentenyladenine (2iP) and gibberellic acid (GA3). A combination of 2iP, BA, and NAA strongly favored induction of shoot buds and shoot proliferation. Pretreatment of shoot cultures at 8°C for 30 days or with 14.4 or 28.9 μM GA3 for 15 days did not improve shoot multiplication. A 16-h photoperiod with photosynthetic photon flux of 30 μmol m-2 s-1 was found to be the optimum light condition for shoot growth and multiplication. Multiple shoots produced well developed root systems within 4 weeks after transfer to a plant growth regulator-free medium containing activated charcoal.

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Abbreviations

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

2iP:

2-isopentenyladenine

MS:

Murashige and Skoog medium

NAA:

1-naphthaleneacetic acid

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Author information

Author notes

  1. Prakash Lakshmanan

    Present address: Tissue Culture Laboratory, Department of Botany, National University of Singapore, Lower Kent Ridge Road 0511, Singapore

Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Thehans Laboratories (S) Pte Ltd, 652 Lim Chu Kang Road, 2471, Singapore

    Prakash Lakshmanan

Authors
  1. Prakash Lakshmanan
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Lakshmanan, P. In vitro establishment and multiplication of Nymphaea hybrid ‘James Brydon’. Plant Cell Tiss Organ Cult 36, 145–148 (1994). https://doi.org/10.1007/BF00048326

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  • DOI: https://doi.org/10.1007/BF00048326

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