Since the first investigations made by Wolff, it has been known that bone adapts to mechanical load. The mechanisms which guide the reaction of osteoblasts and osteoclasts to load are still insufficiently known. In situ hybridization (ISH) allows the detection of intracellular gene transcripts. Therefore, the ISH technique was further developed to allow the detection of pro-alpha 1 (I) collagen gene transcripts on undecalcified bone surfaces. Additionally, this new technique was combined with the tartrate resistant acid phosphatase technique. The combination of the two methods allows the detection of forming and resorbing cells on the same undecalcified bone surface. In addition, a new animal model was developed to study the reaction of bone to mechanical load. This model mimics the situation of bone implants (e.g. hip prosthese), which is a static situation which is dynamically loaded by the action of the patient.
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ROUGHNESS RT defined according to DIN 4768 and DIN 4762/1E (Rauhtiefe), Deutsches Institut für Normung, 10787 Berlin, FRG.
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Department of Traumatology and Reconstructive Surgery andInstitute of Pathology, Klinikum Steglitz, Freie Universität Berlin
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Voigt, C.F., Peljak, P., Müller-Mai, C. et al. Topography of forming and resorbing cells on endosteal surfaces of the rabbit humerus by double-staining with in situ hybridization and tartrate-resistant acid phosphatase reaction: a new model to study the bone reaction to loading. J Mater Sci: Mater Med 6, 279–283 (1995). https://doi.org/10.1007/BF00120271
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DOI: https://doi.org/10.1007/BF00120271