The use of hexamethylene diisocyanate (HMDIC) as a crosslinking agent for dermal sheep collagen (DSC) was studied. Because HMDIC is only slightly water soluble, a surfactant was used to obtain a clear and micellar crosslinking solution and to promote the penetration of HMDIC in the DSC matrix. Using optimized conditions treatment of non-crosslinked DSC (N-DSC) with HMDIC (H-DSC) increased the shrinkage temperature (T s) of N-DSC from 56°C to 74°C for H-DSC. A linear relation between the decrease in free amine group content and the increase in T s was observed. Crosslinking with HMDIC did not influence the tensile strength of the N-DSC samples but increased the elongation at break from 141% to 163% and decreased the high-strain modulus from 26 MPa to 16 MPa for the H-DSC samples, respectively.
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Olde Damink, L.H.H., Dijkstra, P.J., Van Luyn, M.J.A. et al. Crosslinking of dermal sheep collagen using hexamethylene diisocyanate. J Mater Sci: Mater Med 6, 429–434 (1995). https://doi.org/10.1007/BF00120286
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DOI: https://doi.org/10.1007/BF00120286