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The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata

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Various replicates of collagen substrata were prepared to study the attachment, growth and spreading of baby hamster kidney (BHK) cells. Cell attachment was measured in both the presence and absence of serum. Spreading and growth did not occur in the absence of serum. Attachment to fibrous collagen was less than that found with glass, rat-tail tendon collagen or films prepared from pepsin-solubilized collagen (PS-collagen). Incorporation of hyaluronate, heparin and protamine sulphate into the fibrous collagen and the acetylation of fibrous collagen had little effect. However, incorporation of chondroitin sulphate or chemical modification of fibrous collagen by either methylation or succinylation increased BHK cell attachment. In the absence of serum, the attachment to collagen, acetylated collagen and collagen composites was reduced. The reduction in attachment was marked with fibrous collagen and gelatin films, but less so with collagen composites, acetylated collagen, rat-tail tendon and PS-collagen films. Interestingly, attachment to succinylated collagen and methylated collagen was largely unaffected by the absence of serum, and possible reasons for this are discussed. Cell shape measurements showed decreased spreading of BHK cells on chemically modified collagen films, especially on gelatin films and dried PS-collagen gels. Cell shape and spreading on PS-collagen, rat-tail tendon collagen and collagen-composite films was found to be similar to that on fibrous collagen. BHK cell growth on fibrous collagen, chemically modified collagens, collagen composites, rat-tail tendon and PS-collagen films was similar to that found on plastic tissue culture substrate. Denaturation of fibrous collagen resulted in decreased growth, and BHK cell growth was markedly reduced on PS-collagen gels and dried gels.

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References

  1. M. CHVAPIL, R. L. KONENTHAL and W.VAN WINKLE, in “Reviews of connective tissue research”, Vol. 6, edited by D. A. HALL and D. S. JACKSON (Academic Press, London, 1973) p. 1.

    Google Scholar 

  2. D. J. MICHAELI, E. BENJAMIN and D. Y. K. LEUNG, Immunocytochemistry 8 (1971) 1.

    Google Scholar 

  3. D. J. ETHERINGTON, I. A. SILVER and D. K. J. RESTALL, Brit. J. Exp. Pathol. 60 (1979) 549.

    Google Scholar 

  4. K. STENZEL, T. MIYATA and A. RUBIN, Ann. Rev. Biophys. Bioengng 3 (1974) 231.

    Google Scholar 

  5. T. ELSDALE and J. BARD, J. Cell Biol. 54 (1972) 626.

    Google Scholar 

  6. S. L. SCHOR, J. Cell Biol. 41 (1980) 159.

    Google Scholar 

  7. H. K. KLEINMANN, R. J. KLEBE and G. R. MARTIN, J. Cell Biol. 88 (1981) 473.

    Google Scholar 

  8. J. YANG and S. NANDI, Int. Rev. Cytol. 81 (1983) 249.

    Google Scholar 

  9. C. J. DOILLON and F. H. SILVER, Biomaterials 7 (1986) 3.

    Google Scholar 

  10. C. J. DOILLON, F. H. SILVER and R. A. BERG, Biomaterials 8 (1987) 195.

    Google Scholar 

  11. C. J. DOILLON, A. J. WASSERMAN, R. A. BERG and F. H. SILVER, Biomaterials 9 (1988) 91.

    Google Scholar 

  12. M. CHVAPIL, in “Biology of collagen”, Vol. 22, edited by A. Viidik and J. Vuust (Academic Press, London, 1980) p. 313.

    Google Scholar 

  13. M. HATSUOKA, M. SEIKI, K. SASAKI and A. KASHII, Thromb. Res. 42 (1986) 407.

    Google Scholar 

  14. S. A. SAROFF, A. T. CHASENS, S. F. EISEN and S. H. LEVEY, J. Periodont. 53 (1982) 425.

    Google Scholar 

  15. M. D. STEIN, C. M. SALLKIN, A. L. FREEDMAN and V. GLUSHKO, J. Periodont. 56 (1985) 35.

    Google Scholar 

  16. J. H. C. VOORMOLLEN, J. RINGERS, G. T. A. M. BOTS, A.VAN DER HENDE and J. HERMANS, Neurosurgery 20 (1987) 702.

    Google Scholar 

  17. K. E. BURKE, G. NAUGHTON and N. A. CASSAI, Annls Plast. Surg. 14 (1985) 515.

    Google Scholar 

  18. C. N. FORD and D. BLESS, Laryngoscope 96 (1986) 863.

    Google Scholar 

  19. F. DELUSTRO, S. T. SMITH, J. SUNDSMO, G. SALEM, S. KINCAID and L. ELLINGSWORTH, Plast. Reconstr. Surg. 79 (1987) 581.

    Google Scholar 

  20. A. M. KLIGMAN and R. C. ARMSTRONG, J. Dermatol. Surg. Oncol. 12 (1986) 351.

    Google Scholar 

  21. F. M. KAMER and M. M. CHURUKIAN, Arch. Otolaryngol. 110 (1984) 93.

    Google Scholar 

  22. J. M. MCPHERSON, J. S. SAWAMURA and R. ARMSTRONG. J. Biomed. Mater. Res. 20 (1986) 93.

    Google Scholar 

  23. S. J. STEGMAN, S. CHU, K. BENSCH and R. A. ARMSTRONG, Arch. Dermatol. 123 (1987) 1644.

    Google Scholar 

  24. M. CHVAPIL, J. Biomed. Mater. Res. 16 (1982) 245.

    Google Scholar 

  25. B. A. PRUITT and N. S. LEVINE, Arch. Surg. 119 (1984) 312.

    Google Scholar 

  26. I. V. YANNAS and J. F. BURKE, J. Biomed. Mater. Res. 14 (1980) 65.

    Google Scholar 

  27. R. SCOTT, R. B. BARAZA, S. D. GORHAM, I. MCGREGOR and D. A. FRENCH, Brit. J. Urol. 58 (1986) 203.

    Google Scholar 

  28. T. M. ACHIBANA, G. R. NAGAMATSU and J. C. ALDONIZO. J. Urol. 133 (1985) 866.

    Google Scholar 

  29. R. SCOTT, R. MOHAMED, S. D. GORHAM, D. A. FRENCH, M. J. MONSOUR, A. SHIVAS and T. HYLAND, Brit. J. Urol. 62 (1988) 26.

    Google Scholar 

  30. E. RUOSLAHTI, E. G. HAYMAN and M. D. PIERSCHBACHER, Arteriosclerosis 5 (1985) 581.

    Google Scholar 

  31. S. KASAI, T. MIYATA, T. KUNIMOTO and K. NITTA, Biomed. Res. 4 (1983) 147.

    Google Scholar 

  32. S. SRIVASTAVA, S. D. GORHAM, D. A. FRENCH, A. A. SHIVAS and J. M. COURTNEY, Biomaterials 11 (1990) 155.

    Google Scholar 

  33. S. SRIVASTAVA, S. D. GORHAM and J. M. COURTNEY, Biomaterials 11 (1990) 162.

    Google Scholar 

  34. E. G. MILLER and R. K. RHODES, Methods Enzymol. 82 (1982) 65.

    Google Scholar 

  35. S. KASSAI, T. AKAIKE, T. KUNIMOTO and K. NITTA, J. Biomed. Mater. Res. 18 (1984) 243.

    Google Scholar 

  36. G. E. MEANS and R. E. FEENEY, “Chemical modification of proteins” (Holden-Day, San Francisco, 1971).

    Google Scholar 

  37. C. L. WANG, T. MIYATA, B. WEKSLER, A. L. RUBIN and K. H. STENZEL, Biochim. Acta. 544 (1978) 568.

    Google Scholar 

  38. A. V. HOFFBRAND and S. M. LEWIS, “Postgraduate haematology, 1982” (Heinemann Medical, London, 1982).

    Google Scholar 

  39. E. ENGVALL and E. RUOSLAHTI, Int. Rev. Cancer 20 (1977) 1.

    Google Scholar 

  40. J. G. EDWARDS, R. T. ROBSON and G. CAMPBELL, J. Cell Sci. 87 (1987) 657.

    Google Scholar 

  41. A. F. BROWN, V. DUGINA, G. A. DUNN and J. M. VASILIEV, Cell Biol. Int. Rep. 13 (1989) 357.

    Google Scholar 

  42. F. GRINNELL and D. MINTER, Proc. Natn. Acad. Sci. USA 75 (1978) 4408.

    Google Scholar 

  43. S. SCHOR and J. COURT, J. Cell Sci. 38 (1979) 267.

    Google Scholar 

  44. J. J. TOMASEK, E. D. HAY and K. FUJIWARA, Devel. Biol. 92 (1982) 107.

    Google Scholar 

  45. F. GRINNELL and M. H. BENNETT, J. Cell Sci. 48 (1981) 19.

    Google Scholar 

  46. F. GRINNELL, M. FELD and D. F. MINTER, Cell 19 (1980) 517.

    Google Scholar 

  47. F. GRINNELL, in “Growth and maturation factors”, Vol. 1, edited by G. Guroff (Wiley, New York) p. 267.

  48. S. SCHOR and J. COURT, J. Cell Sci. 38 (1979) 267.

    Google Scholar 

  49. F. GRINNELL, H. FUKANIZU, P. PAWELEH and S. NAKAGAWA, Exp. Cell. Res. 181 (1989) 483.

    Google Scholar 

  50. P. A. HARPER, P. BROWN and R. L. JULIANO, J. Cell Sci. 63 (1983) 287.

    Google Scholar 

  51. J. L. R. BROWN and R. AKESON, Devel. Biol. 96 (1983) 304.

    Google Scholar 

  52. R. W. RHUDY and J. M. MCPHERSON, J. Cell. Physiol. 137 (1988) 185.

    Google Scholar 

  53. I. RUCKER, R. KETTREY and L. D. ZELEKNICK, Soc. Exp. Biol. Med. 139 (1981) 749.

    Google Scholar 

  54. D. GOSPODAROWICZ, D. DELGADO and I. VLODAVSKY, Proc. Natn. Acad. Sci. USA 77 (1980) 4094.

    Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology, University of Glasgow, G12 8QQ, Glasgow, UK

    G. G. Reid

  2. Johnson and Johnson Biopolymer Research Group, Stirling University, Innovation Park, FK9 4NE, Stirling, UK

    S. D. Gorham

  3. Yamanouchi Research Institute, UK, Littlemore Hospital, OX4 4XN, Oxford, UK

    J. M. Lackie

Authors
  1. G. G. Reid
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  2. S. D. Gorham
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  3. J. M. Lackie
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Reid, G.G., Gorham, S.D. & Lackie, J.M. The attachment, spreading and growth of baby hamster kidney cells on collagen, chemically modified collagen and collagen-composite substrata. J Mater Sci: Mater Med 4, 201–209 (1993). https://doi.org/10.1007/BF00120393

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