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Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae

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Summary

The enriched medium based on yeast nitrogen basc(YNB)increased hirudin synthesis and secretion in rccombinant Saccharomyces cerevisiae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudin/l but supplementation of yeast extract increased the final hirudin concentration to 461mg hirudin/l. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous culuture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium.

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Authors and Affiliations

  1. Department of Food Science & Technology, Research Center for New Bio-Materials in Agriculture, Seoul National University, Seoul, Korea

    Chi-Min Choi, Myoung-Dong Kim, Sang-Ki Rhee & Jin-Ho Seo

  2. Korea Research Institute of Bioscience & Biotechnology, Korea

    Sang-Ki Rhee

Authors
  1. Chi-Min Choi
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  2. Myoung-Dong Kim
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  3. Sang-Ki Rhee
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  4. Jin-Ho Seo
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Choi, CM., Kim, MD., Rhee, SK. et al. Effects of medium composition on hirudin production in recombinant Saccharomyces cerevisiae . Biotechnol Lett 18, 1129–1132 (1996). https://doi.org/10.1007/BF00128579

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  • DOI: https://doi.org/10.1007/BF00128579

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