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Optimization of β-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose

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Summary

Purified β-Glucosidase from Fusarium oxysporum catalysed the hydrolysis and transglycosylation reactions in the presence of cellobiose and gentiobiose. The product of the latter reaction was mainly a triose. The time of incubation, pH and substrate concentration for transglycosylation reaction were optimised. Under optimal conditions, the concentration of glucose and triose reached approximately 15–20 % of the initial substrate concentration. These results suggested that β-glucosidase from F.oxysporum is an ideal enzyme for the synthesis of triose in reasonable quantities.

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Authors and Affiliations

  1. Department of Protein Engineering, Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, RG6 2EF, Reading, UK

    Paul Christakopoulos, Peter W. Goodenough & Mahalingeshwara K. Bhat

  2. Department of Chemical Engineering, National Technical University of Athens, 5 Iroon Poltechniou Ave, Zografou Campus, 15 700, Athens, Greece

    Dimitris Kekos & Basil J. Macris

Authors
  1. Paul Christakopoulos
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  2. Dimitris Kekos
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  3. Basil J. Macris
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  4. Peter W. Goodenough
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  5. Mahalingeshwara K. Bhat
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Christakopoulos, P., Kekos, D., Macris, B.J. et al. Optimization of β-glucosidase catalysed synthesis of trisaccharides from cellobiose and gentiobiose. Biotechnol Lett 16, 587–592 (1994). https://doi.org/10.1007/BF00128605

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  • DOI: https://doi.org/10.1007/BF00128605

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