Summary
A white rot fungus was isolated from rotted leaves and identified as Pestalotiopsis sp. KCTC 8637P. It produced a flocculant biopolymer. A flocculant was partially purified from the culture broth by series of precipitations with 95% ethanol and named as Pestan. The components of Pestan were consisted of glucose : glucosamine : glucuronic acid : rhamnose with a approximately molar ratio of 100:3.5:1.6:1.3.
In kaolin suspension(final concentration was 4,800 mg/l), the highest flocculating activity was attained at the biopolymer flocculant concentration of 1 mg/l . The flocculating activity was observed most highest by the addition of cationic solutions, especially 8mM CaCl2 · 2H2O or 8mM FeCl3. The thermal stability of Pestan was sustained up to 70 °C.
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Kwon, G.S., Moon, S.H., Hong, S.D. et al. A novel flocculant biopolymer produced by Pestalotiopsis sp. KCTC 8637P. Biotechnol Lett 18, 1459–1464 (1996). https://doi.org/10.1007/BF00129355
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DOI: https://doi.org/10.1007/BF00129355