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Protease production by a thermophilic Bacillus species (P-001A) which degrades various kinds of fibrous proteins

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Abstract

An aerobic Gram-positive sporeforming bacterium was isolated from an alkaline hot spring at Wondo Genet, Ethiopia. In an optimized culture medium it produced maximum activity of protease at 55°C and pH 9.5. The protease activity against casein was 65 units/ml. Enzyme activity was detected between 30–70°C and pH of 4.5–11.5. The enzyme had a half-life of 55 and 30 min at 60° and 70°C, respectively. The isolate hydrolysed 90, 60 and 50% of the skin, feather and horn used in the optimized medium within 120 h.

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Authors and Affiliations

  1. Department of Biology, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia

    Atalo K. & Gashe B. A.

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  1. Atalo K.
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  2. Gashe B. A.
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Atalo, K., Gashe, B.A. Protease production by a thermophilic Bacillus species (P-001A) which degrades various kinds of fibrous proteins. Biotechnol Lett 15, 1151–1156 (1993). https://doi.org/10.1007/BF00131207

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