Abstract
A H+-selective liquid membrane microelectrode was prepared and used to measure the pH profile evolution during colonization of gel beads immobilizing Lactobacillus helveticus in a whey permeate medium. A large pH gradient was observed in a highly active periferal layer thickness that decreased from 500 to 300 μm for an immobilized cell population that increased from 5.8 × 109 to 3.1 × 1010 CFU/g. The flat pH profile (pH 4.4) in the central part of the bead was attributed to a high concentration of the inhibitory undissociated form of lactic acid.
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Massen, F., Lacroix, C. & Paquin, C. Direct measurement of pH profiles in gel beads immobilizing Lactobacillus helveticus using a pH sensitive microelectrode. Biotechnol Tech 8, 551–556 (1994). https://doi.org/10.1007/BF00152144
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DOI: https://doi.org/10.1007/BF00152144