Summary
Endo-xylanase from T. viride has been purified 4.2 fold by precipitation with a commercially available enteric polymer Eudragit S-100. Electrophoretic analysis also indicated removal of contaminant proteins. The enzyme could be recovered in more than 89% yield. The binding of the enzyme to the polymer was predominantly by electrostatic interaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, M.J., and Poutanen, K. (1992). In: Xylans and Xylanases, J. Visser, G. Beldman, M.A. Kusters-van Someren, and A.G.J. Voragen, eds. pp. 171–186, Amsterdam: Elsevier.
Coughlan, M.P., and Hazelwood G.P. (1993). Biotechnol. Appl. Biochem. 17, 259–289.
Debeire-Gosselin, M., Loonis, M., Samain, E. and Debeire, P. (1992). In: Xylans and Xylanases, J. Visser, G. Beldman, M.A. Kusters-van Someren, and A.G.J. Voragen, eds. pp. 463–466, Amsterdam: Elsevier.
Debeire-Gosselin, M., Touzel, J. P., and Debeire, P. (1992a). In: Xylans and Xylanases, J. Visser, G. Beldman, M.A. Kusters-van Someren, and A.G.J. Voragen, eds. pp. 471–474, Amsterdam: Elsevier.
Fisk, R. S. and Simpson, C. (1993) In: Stability and Stabilization of Enzymes, W.J.J. van den Tweel, A. Harder, R. M. Buitelaar, eds. pp.323–328, Amsterdam: Elsevier.
Fujii, M. and Taniguchi, M. (1991). Trends Biotechnol. 9, 191–196.
Ghose, S., and Mattiasson, B. (1993). In: Thermostability of Enzymes, M.N. Gupta, ed. pp. 24–42, New Delhi: Springer Verlag.
Guoqiang, D., Kaul, R., and Mattiasson, B. (in press). Bioseparation.
Hames, B.D. (1986). In: Gel Electrophoresis of Proteins — a practical Approach, B.D. Hames and D. Rickwood, eds. pp. 1–86, Oxford: IRL press.
Kamihara, M., Kaul, R., and Mattiasson B. (1992). Biotechnol. Bioeng. 40, 1381–1387.
Smith, P.K., Krohn, R. I. and Hermanson, E. K. (1985). Anal. Biochem. 150, 76–85.
Visser, J., Beldman G., Kusters-van Someren, M.A., and Voragen, A.G.J., eds. (1992). Xylans and Xylanases, Amsterdam: Elsevier.
Wong, K.K.Y., and Saddler, J.N. (1992).In: Xylans and Xylanases, J. Visser, G. Beldman, M.A. Kusters-van Someren, and A.G.J. Voragen, eds. pp. 171–186, Amsterdam: Elsevier.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gupta, M.N., Guoqiang, D., Kaul, R. et al. Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S 100. Biotechnol Tech 8, 117–122 (1994). https://doi.org/10.1007/BF00152851
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00152851