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Increasing glucose determination range by flow injection analysis (FIA) using glucose oxidase immobilised on polyaniline

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Biotechnology Techniques

Abstract

A Flow Injection Analysis (FIA) for glucose using glucose oxidase (E.C.1.1.3.4) was developed. The enzyme was immobilised on polyaniline chemically synthetized on internal surface of silicon tube (2.0 mm of diameter and length from 1 to 5 meters), using glutaraldehyde as a bifunctional agent. The system was able to measure glucose at levels from 5 to 500mM with a response time of 2min using 250μl of sample. The system has been operated satisfactorily for one month with more than 300 assays with loss of 60% of activity.

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Authors and Affiliations

  1. Departamento de Bioquímica, Universidade Federal de Pernambuco, Av Prof. Moraes Rego, S/N. Cidade Universitária, CEP 50730-910, Recife, PE, Brazil

    V. Leite, E. H. M. Melo & J. L. Lima Filho

  2. Departamento de Engenharia Química-CT, Universidade Federal de Pernambuco, Av Prof. Moraes Rego, S/N. Cidade Universitária, CEP 50730-910, Recife, PE, Brazil

    V. L. da Silva

  3. Departamento de Química Fundamental-L.Q.E.S./CCEN, Universidade Federal de Pernambuco, Av Prof. Moraes Rego, S/N. Cidade Universitária, CEP 50730-910, Recife, PE, Brazil

    W. M. Azevedo

  4. Laboratório de Imunopatologia Keizo Asami/LIKA/Universidade Federal de Pernambuco, Av Prof. Moraes Rego, S/N. Cidade Universitária, CEP 50730-910, Recife, PE, Brazil

    V. Leite, E. H. M. Melo & J. L. Lima Filho

Authors
  1. V. Leite
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  2. V. L. da Silva
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  3. W. M. Azevedo
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  4. E. H. M. Melo
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  5. J. L. Lima Filho
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Leite, V., da Silva, V.L., Azevedo, W.M. et al. Increasing glucose determination range by flow injection analysis (FIA) using glucose oxidase immobilised on polyaniline. Biotechnol Tech 8, 133–136 (1994). https://doi.org/10.1007/BF00152854

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  • DOI: https://doi.org/10.1007/BF00152854

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