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Introduction of foreign DNA into Chlorella saccharophila by electroporation

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Biotechnology Techniques

Summary

Plasmid, pBI221, was introduced into protoplasts of Chlorella saccharophila c-211-1a prepared from the cells in the stationary phase by electroporation. Transient expression of the introduced plasmid was observed under a field strength of between 600 and 900 V/cm, and a pulse duration of around 400 ms, where high membrane permeability to 70-kDa FITC-dextran was ascertained.

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Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, 227, Yokohama, Japan

    Masato Maruyama, Irena Horáková, Hiroyuki Honda, Xin-hui Xing, Naohiro Shiragami & Hajime Unno

  2. Oral Care Research Laboratory, Lion Limited Corporation, Japan

    Masato Maruyama

  3. U'stav Plicni'ch Nemoci', Praha, The Czech Republic

    Irena Horáková

  4. Dept of Biotechnology, Faculty of Engineering, Nagoya University, Nagoya, Japan

    Hiroyuki Honda

Authors
  1. Masato Maruyama
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  2. Irena Horáková
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  3. Hiroyuki Honda
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  4. Xin-hui Xing
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  5. Naohiro Shiragami
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  6. Hajime Unno
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Maruyama, M., Horáková, I., Honda, H. et al. Introduction of foreign DNA into Chlorella saccharophila by electroporation. Biotechnol Tech 8, 821–826 (1994). https://doi.org/10.1007/BF00152891

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  • DOI: https://doi.org/10.1007/BF00152891

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