Summary
Immobilization of Candida rugosa lipase (Amano AY-30) by adsorption onto Celite 545 led to a markedly improved performance of the enzyme: Firstly, the enzyme was almost completely resistant to deactivation by acetaldehyde, which is liberated as unavoidable by-product in acyl-transfer reactions with vinyl esters, and secondly, the enantioselectivity was enhanced up to three-fold.
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Kaga, H., Siegmund, B., Neufellner, E. et al. Stabilization of Candida lipase against acetaldehyde by adsorption onto celite. Biotechnol Tech 8, 369–374 (1994). https://doi.org/10.1007/BF00154305
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DOI: https://doi.org/10.1007/BF00154305