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Production of neutral protease from Aspergillus oryzae by a novel cultivation method on a microporous membrane

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Biotechnology Techniques

Summary

A novel cultivation method using a microporous membrane (membrane-surface liquid culture) was developed, in which moulds are grown on the membrane surface with its opposite side being in contact with a liquid medium. The amount of neutral protease from Aspergillus oryzae produced was more than 10 times higher than that produced by the conventional liquid culture.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka, 700, Okayama, Japan

    A. Yasuhara, A. Ogawa, T. Tanaka, T. Sakiyama & K. Nakanishi

Authors
  1. A. Yasuhara
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  2. A. Ogawa
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  3. T. Tanaka
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  4. T. Sakiyama
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  5. K. Nakanishi
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Yasuhara, A., Ogawa, A., Tanaka, T. et al. Production of neutral protease from Aspergillus oryzae by a novel cultivation method on a microporous membrane. Biotechnol Tech 8, 249–254 (1994). https://doi.org/10.1007/BF00155416

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  • DOI: https://doi.org/10.1007/BF00155416

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