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Monitoring biomass and glycerol in an Escherichia coli fermentation using near-infrared spectroscopy

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Biotechnology Techniques

Summary

Near-infrared spectroscopy was used to determine biomass and glycerol concentrations in E.coli whole broth fermentation samples. For dry cell weight, a standard error of prediction (SEP) of 0.2 g/L and correlation coefficient (r) of 0.991 were obtained. The SEP and r for glycerol, carbon nutrient, were 0.3 g/L and 0.979. respectively. Off-line analysis was accomplished within 2 minutes of sampling and therefore provides the opportunity to monitor fermentations quickly enough to permit in-process development and troubleshooting.

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Authors and Affiliations

  1. Department of Biotechnology, Alberta Research Council, 250 Karl Clark Rd., T6C 3X1, Edmonton, Alberta, Canada

    G. Macaloney, I. Draper & B. G. Thompson

  2. NIRSystems Inc., 127 Farquharson Bldg., York University Campus, 4700 Keele St., M3J 1P3, Toronto, Ontario, Canada

    J. W. Hall

  3. Department of Clinical Biochemistry, Mount Sinai Hospital, 600 University Ave., M5G 1X5, Toronto, Ontario, Canada

    J. W. Hall

  4. Biostar Inc., Box 1000. Sub P.O. #6, S7N 0W0, Saskatoon, Saskatchewan, Canada

    M. J. Rollins

  5. Department of Bioscience and Biotechnology, University of Strathclyde, 204 George St., G1 1XW, Glasgow, Scotland

    B. McNeil

Authors
  1. G. Macaloney
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  2. J. W. Hall
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  3. M. J. Rollins
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  4. I. Draper
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  5. B. G. Thompson
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  6. B. McNeil
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Macaloney, G., Hall, J.W., Rollins, M.J. et al. Monitoring biomass and glycerol in an Escherichia coli fermentation using near-infrared spectroscopy. Biotechnol Tech 8, 281–286 (1994). https://doi.org/10.1007/BF00155422

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  • DOI: https://doi.org/10.1007/BF00155422

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