Abstract
We have studied the influence of low concentrations of 6-benzyladenine on growth limitation, in order to preserve coffee germplasm through a microcutting collection. Concentrations of 0 μM, 1.3 μM and 4.4 μM were compared in four species: Coffea congensis, C. canephora, C. liberica and C. racemosa. After six months, microcutting behaviour varied between the different treatments, and a species effect was observed. The slow growing species (C. liberica and C. congensis) needed 1.3 μM; the others coffee species (C. canephora and C. racemosa) exhibited moderate caulogenesis on 6-benzyladenine-free medium. Zero and low concentrations did not affect survival rates. In conclusion 1.3 μM seems most appropriate for conserving all four species.
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Abbreviations
- BA:
-
6-benzyladenine
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Bertrand-Desbrunais, A., Noirot, M. & Charrier, A. Minimal growth in vitro conservation of coffee (Coffea spp.). Plant Cell Tiss Organ Cult 27, 333–339 (1991). https://doi.org/10.1007/BF00157599
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DOI: https://doi.org/10.1007/BF00157599