Abstract
A study of the toxicity of epinigericin, an antibiotic ionophor, towards the ciliate Tetrahymena pyriformis showed that this molecule stopped cell division, increased cell volume and led to a more basic intracellular pH. The action of epinigericin was probably linked to its function as an ionophor. The ionic selectivity of this molecule is still not known. The raising of the intracellular pH of ciliates by this antibiotic may be linked to its toxic action and its ion-transport mechanism in Tetrahymena. *** DIRECT SUPPORT *** AG903066 00009
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Bamdad M, Grolière CA, Dupy-Blanc J, Cuer A, David L, Tabet JC (1993a) Microbial conversion of ionophorous antibiotic nigericin by ciliate Tetrahymena pyriformis. Eur J Protistol 29:407–415
Bamdad M, Grolière CA, Dupy-Blanc J, David L, Gendraud M (1993b) Change in intracellular pH in Tetrahymena pyriformis GL in relation to the toxicity of the ionophorous antibiotic nigericin. Eur J Protistol 29:230–237
Bamdad M, Grolière CA, Dupy-Blanc J, David L (1994) Toxicity of two pairs of epimeric ionophorous antibiotics towards Tetrahymena pyriformis. Structure-activity relationship. Arch Proctistenkd 144:297–307
Barrans Y, Alleaume M, David L (1980) Complexe de sodium de I'ionophore nigéricine. Acta Cristallogr B36:936–938
Berrada R, Dauphin G, David L (1987) Epinigericin, a new polyether carboxylic antibiotic. Structural determination by 2D NMR methods. J Org Chem 52:2388–2391
Berrada R, Grolière CA, Dupy-Blanc J, David L (1988) Action comparée de deux antibiotiques polyethers carboxyliques épimères (nigéricine et épinigéricine) sur des populations de Tetrahymena pyriformis en croissance exponentielle ou synchronisée. Arch Protistenkd 136:317–326
Bogaerts C, Gomez L, Jouany JP, Jeminet G (1989) Effects of the ionophore antibiotics lasalocid and cationomycin on ruminal fermentation in vitro (RUSITEC). Anim Feed Sci Technol 27:1–15
Bolte J, Demuynck C, Jeminet G, Juillard J, Tissier C (1982) Etude comparée de trois antibiotiques ionophores X.537A (lasalocid), A.23187 (calcimycin) et X.14547A. Complexation des cations IA et IIA. Can J Chem 60:981–989
Connoly G, Kerkut GA (1983) Ion regulation and membrane potential in Tetrahymena and Paramecium. Comp Biochem Physiol 76:1–16
Delort AM, Dauphin G, Goyut J, Jeminet G (1989) Study by NMR of the mode of action of monensin on Streptococcus faecalis de-energized and energized cells. Biochim Biophys Acta 1013:11–20
Dunham PB, Child FM (1961) Ion regulation in Tetrahymena. Biol Bull (woods Hole) 121:129–140
Dupy-Blanc J, Grolière CA, Berrada R, David L (1989) Action comparée de deux antibiotiques ionophores (nigéricine, épinigéricine) sur le teneur en DAN macromoléculaire du cilié Tetrahymena pyriformis GL, en culture synchrome. Eur J Protistol 24:138–144
Dupy-Blanc J, David L, Grolière CA, Bamdad M (1991) Etude autoradiographique de la localisation intracellulaire de la nigéricine chez Tetrahymena pyriformis. Ann Sci Nat Zool 12:1–4
Gachon P, Chaput P, Jeminet G, Juillard J, Morel JP (1975) Grisorixin, an ionophorous antibiotic of the nigericin group. Part IV. Complexation of monovalent cations. J Chem Soc (Perkin II) :907–911
Grolière CA, Dupy-Blanc J, Berrada R, David L (1986–1987) Action comparée de deux antibiotiques polyethers carboxyliques épimères (la nigéricine et l'épinigéricine) sur l'ultrastructure de Tetrahymena pyriformis. Ann Sci Nat Zool 8:119–133
Grolière CA, Dupy-Blanc J, Berrada R, David L (1990) Etude de la toxicité de deux antibiotiques polyethers carboxyliques au cours du cycle cellulaire chez le cilé Teterahymena pyriformis GL. In: sefa (ed) Ecotoxicologie cellulaire. pp 131–133
Hilaire MC, Jouany JP, Gaboyard C, Jeminet G (1989) In vitro study of the effect of different ionophore antibiotics and of certain derivates on rumen fermentation and on protein nitrogen degradation. Reprod Nutr Dev 29:247–257
Liu CM (1982) In: Westley JW (ed) Polyether antibiotics, vol 1. Biology. Dekker, New York, pp 53–54
Nilsson JR (1977) Effects of dimethylsulfoxide on T. pyriformis GL. Fine structural changes and their reversibility. J Protozool 24:275–283
Plessner P, Rasmussen L, Zeuthen E (1964) Techniques used in study of synchronous Tetrahymena. In: Zeuthen E (ed) Synchrony in cell division and growth, Intersciences, New York, pp 534–565
Pressmann BC (1973) Properties of ionophore with broad range cation selectivity. Fed Proc 32:1696–1703
Ruff MD (1982) In: Westley JW (ed) Polyether antibiotics, vol 1. Biology. Dekker, New York, pp 303–332
Westley JW (1982) In: Westley JW (ed) Polyether antibiotics, vol 1. Biology. Dekker, New York, pp 177–223
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Bamdad, M., David, L. & Groliére, C.A. Epinigericin toxicity towards Tetrahymena pyriformis GL; changes in cell volume and intracellular pH. Appl Microbiol Biotechnol 44, 206–209 (1995). https://doi.org/10.1007/BF00164503
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DOI: https://doi.org/10.1007/BF00164503