Summary
Growth of Trichoderma harzianum E58 on hemicellulose-rich media, both in batch and fermentor cultures, resulted in independent profiles for the production of xylanase and endoglucanase enzymes. Dramatic differences in the ratio of xylanase to endoglucanase activities were observed among cultures grown on cellulose-rich Solka Floc and xylan. These results indicated that the induction of xylanases and cellulases was likely to be under separate regulatory control. The specific activity and amount of xylanases produced were found to be dependent on the concentration of xylan in the growth media. Growth on oat spelts xylan or the hemicellulose-rich, water-soluble fraction from steam-treated aspenwood (SEA-WS) greatly enhanced the production of xylanases and xylosidase in the culture filtrates. Constitutive levels of xylanase and endoglucanase enzymes were detected during growth of the fungus on glucose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beldman G, Voragen AGJ, Rombouts FM, Searle-van-Leeuwen MF, Pilnik W (1988) Specific and nonspecific glucanases from Trichoderma viride. Biotechnol Bioeng 31:160–167
Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286–290
Biely P, MacKenzie CR, Schneider H (1988) Production of acetyl xylan esterase by Trichoderma reesei and Schizophyllum commune. Can J Microbiol 34:767–772
Brownell HH, Saddler JN (1987) Steam pretreatment of lignocellulosic material for enhanced enzymatic hydrolysis. Biotechnol Bioeng 29:228–235
Chaudhary K, Tauro P (1986) Synthesis of xylanase by Trichoderma reesei. MIRCEN J 2:399–403
Dekker RFH (1983) Bioconversion of hemicellulose: aspects of hemicellulase production by Trichoderma reesei QM9414 and enzymatic saccharification of hemicellulose. Biotechnol Bioeng 25:1127–1146
Hrmová M, Biely P, Vršanská M (1986) Specificity of cellulase and β-xylanase induction in Trichoderma reesei QM9414. Arch Microbiol 144:307–311
Knapp JS, Legg M (1986) The effect of different cellulosic growth substrates and pH on the production of cellulolytic enzymes by Trichoderma reesei. J Appl Bacteriol 61:319–329
Lowry OH, Rosebrough NJ, Farr AC, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
MacKenzie CR, Bilous D, Schneider H, Johnson KG (1987) Induction of cellulolytic enzyme systems in Streptomyces spp. Appl Environ Microbiol 53:2835–2839
Miller GL (1959) Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Anal Chem 31:426–428
Montenecourt BS, Eveleigh DE (1977) Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Appl Environ Microbiol 34:777–784
Paice MG, Jurasek L, Bernier R Jr (1988) Viscosity-enhancing bleaching of hardwood kraft pulp with xylanase from a cloned gene. Biotechnol Bioeng 32:235–239
Poutanen K, Ratto M, Puls J, Viikari L (1987) Evaluation of different microbial xylanolytic systems. J Biotechnol 6:49–60
Saddler JN, Mes-Hartree M (1984) The enzymatic hydrolysis and fermentations of pretreated wood substrates. Biotechnol Adv 2:161–181
Saddler JN, Hogan CM, Chan MK-H, Louis-Seize G (1982) Ethanol fermentation of enzymatically hydrolyzed pretreated wood fractions using Trichoderma cellulases, Zymomonas mobilis and Saccharomyces cerevisiae. Can J Microbiol 28:1311–1319
Saddler JN, Chan MKH, Mes-Hartree M, Breuil C (1987) Cellulase production and hydrolysis of pretreated lignocellulosic substrates. In: Moo-Young M (ed) Biomass conversion technology, principles and practice. Pergamon Press, Oxford, pp 149–165
Senior DJ, Mayers PR, Miller D, Sutcliffe R, Tan LUL, Saddler JN (1988) Selective solubilization of xylan in pulp using a purified xylanase from Trichoderma harzianum. Biotechnol Lett 10:907–912
Senior DJ, Mayers PR, Saddler JN (1989) Production and purification of xylanases. In: Lewis NG, Paice MG (eds) Biogenesis and biodegradation of plant cell wall polymers, 1988 ACS Symposium Series. ACS Symposium Series No. 399, American Chemical Society, Washington, pp 641–654
Sprey B, Lambert C (1983) Titration curves from Trichoderma reesei: demonstration of a cellulase-xylanase-β-glucosidase-containing complex. FEMS Microbiol Lett 18:217–222
Tan LUL, Chan MKH, Saddler JN (1984) A modification of the Lowry method for detecting protein in media containing lignocellulosic substrates. Biotechnol Lett 6:199–204
Tan LUL, Yu EKC, Louis-Seize GW, Saddler JN (1987) Inexpensive, rapid procedure for bulk purification of cellulase-free β-1,4-d-xylanase of high specific activity. Biotechnol Bioeng 30:96–100
Thaker AJ, Ray RM, Patel HC (1986) Xylanase production by the fungus Trichoderma longibrachiatum and enhancement in enzymatic saccharification of cellulosic wastes. Ind J Exp Biol 24:659–662
Yu EKC, Tan LUL, Chan MKH, Deschatelets L, Saddler JN (1987) Production of thermostable xylanase by a thermophilic fungus Thermoascus aurantiacus. Enzyme Microb Technol 9:16–24
Wheelwright SM (1987) Designing downstream processes for large-scale protein purification. Biotechnology 5:789–793
Author information
Authors and Affiliations
Additional information
Offprint requests to: D. J. Senior
Rights and permissions
About this article
Cite this article
Senior, D.J., Mayers, P.R. & Saddler, J.N. Xylanase production by Trichoderma harzianum E58. Appl Microbiol Biotechnol 32, 137–142 (1989). https://doi.org/10.1007/BF00165877
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00165877