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Purification and characterization of mono-and diacylglycerol lipase isolated from Penicillium camembertii U-150

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Summary

A novel enzyme hydrolysing mono- and diacylglycerol was found in the culture filtrate of an isolated fungus, Penicillium camembertii. The enzyme was separated into two forms (A- and B-enzyme) with almost the same molecular weight (37,000–39,000), amino acid composition and identical N-terminal amino acid sequence. B-Enzyme, a major component, was purified approximately 210-fold with an activity yield of 2.6%. The B-enzyme was specific to mono- and diacylglycerols and hydrolysed long-chain monoacylglycerols most efficiently. Triacylglycerols were completely inert as substrates for the enzyme. The B-enzyme preferred to attack α-position to β-position of monoacylglycerol, but showed no stereospecificity on mono- and diacylglycerol. Both Fe3+ and Hg2+ inhibited B-enzyme activity significantly.

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References

  • Akesson B, Gronowitz S, Herslof B, Michelsen P, Olivecrona T (1983) Stereospecificity of different lipases. Lipids 18:313–318.

    Google Scholar 

  • Azuma T (1977) JIS Z 8721 junkyo Hyojun Shikiryo, Nihon Kikaku Kyokai, Tokyo.

    Google Scholar 

  • Brady L, Brazozowski AM, Derewenda ZS, Dodson G, Tolley S, Turkenberg JP, Christiansen L, Huge-Jensen B, Norskov L, Thim L, Menge U (1990) A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343:767–770.

    Google Scholar 

  • Chopineau J, McCafferty FD, Therisod M, Klibanov AM (1988) Production of biosurfactants from sugar alcohols and vegetable oils catalyzed by lipase in a nonaqueous medium. Biotechnol Bioeng 31:208–214.

    Google Scholar 

  • Hoq MM, Yamane T, Shimizu S, Funada T, Ishida S (1985) Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor. J Am Oil Chem Soc 62:1016–1021.

    Google Scholar 

  • Huge-Jensen B, Galluzzo DR, Jensen RG (1987) Partial purification and characterization of free and immobilized lipases from Mucor miehei. Lipids 22:559–565.

    Google Scholar 

  • Ikeda Y, Okumura K, Fujii S (1977) Purification and characterization of rat liver microsomal monoacylglycerol lipase in comparison to the other esterases. Biochim Biophys Acta 488:128–139.

    Google Scholar 

  • Iwai M, Tsujisaka Y (1984) Fungal lipase. In: Borgström B, Brockman HL (eds) Lipases. Elsevier Science Publishers, Amsterdam, pp 443–469.

    Google Scholar 

  • Kirchner G, Scollar MP, Klivanov AM (1985) Resolution of racemic mixtures via lipase catalysis in organic solvents. J Am Chem Soc 107:7072–7076.

    Google Scholar 

  • Linfield WM, O'Brien DJ, Serata S, Barauskas RA (1984) Lipid-lipase interactions. I. Fat splitting with lipase from Candida rugosa. J Am Oil Chem Soc 61:1067–1071.

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275.

    CAS  PubMed  Google Scholar 

  • Nagao A, Kito M (1989) Synthesis of O-acyl-l-homoserine by lipase. J Am Oil Chem Soc 66:710–713.

    Google Scholar 

  • Okumura S, Iwai M, Tsujisaka Y (1980) Purification and properties of partial glyceride hydrolase of Penicillium cyclopium M1. J Biochem 87:205–211.

    Google Scholar 

  • Pitt JI (1979) Chapter 4. Isolation, cultivation and preservation, p. 16. Chapter 10. Subgenus Penicillium, p. 316. In: Pitt JI (ed) The Genus Penicillium. Academic Press, London.

    Google Scholar 

  • Raper KB, Thom C (1968) Chapter 8. Section Asymmetrica Raper and Thom. In: Raper KB, Thom C (ed) A manual of the Penicillia. The Williams and Wilkins, Baltimore, p 254.

    Google Scholar 

  • Sugiura M (1984) Bacterial lipases. In: Borgström B, Brockman HL (eds) Lipases. Elsevier Science Publishers, Amsterdam, pp 505–523.

    Google Scholar 

  • Tornqvist H, Belfrage P (1976) Purification and some properties of a monoacylglycerol-hydrolyzing enzyme of rat adipose tissue. J Biol Chem 251:813–819.

    Google Scholar 

  • Winkler FK, D'Arcy A, Hunziker W (1990) Structure of human pancreatic lipase. Nature 343:771–774.

    Google Scholar 

  • Yokozeki K, Yamanaka S, Takinami K, Hirose Y, Tanaka A, Sonomoto K, Fukui S (1982) Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent. Appl Microbiol Biotechnol 14:1–5.

    Google Scholar 

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Author notes

  1. Shotaro Yamaguchi

    Present address: Tsukuba Research Laboratories, Amano Pharmaceutical Co. Ltd., 22 Miyukigaoka, 305, Tsukuba, Ibaragi, Japan

Authors and Affiliations

  1. Research andelopment Division, Amano Pharmaceutical Co., Ltd., Nishiharu-cho, 481, Nishikasugai-gun, Aichi, Japan

    Shotaro Yamaguchi & Tamio Mase

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  1. Shotaro Yamaguchi
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  2. Tamio Mase
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Yamaguchi, S., Mase, T. Purification and characterization of mono-and diacylglycerol lipase isolated from Penicillium camembertii U-150. Appl Microbiol Biotechnol 34, 720–725 (1991). https://doi.org/10.1007/BF00169340

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  • DOI: https://doi.org/10.1007/BF00169340

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