Summary
Pseudomonas sp. CBS3 converted various nitro-aromatic compounds under aerobic resting-cell conditions to the corresponding amino compounds. Mononitro-compounds were reduced to anilines. 1-Chloro-2,4-dinitrobenzene was reduced via the two possible chloronitroanilines to 4-chloro-1,3-diaminobenzene. In the case of 2,4,6-trinitrotoluene, two monoaminodinitrotoluenes and one diaminomononitrotoluene were obtained. In addition to the reduction, in most cases the amines were partially acetylated. In experiments under an argon atmosphere conversion of the nitro-compounds was as fast as under aerobic conditions. Cells of Pseudomonas sp. CBS3 cultivated on complex medium showed higher nitro-reducing activity than those cultivated on mineral salts medium with 4-chlorobenzoate as substrate, which is normally used as medium for this strain. Several other Pseudomonas species (ATCC 4359, ATCC 23937, ATCC 15005, ATCC 17933) also showed nitro-reducing activities. In crude cell-free extracts of Pseudomonas sp. CBS3 an enzyme catalysing the reduction of nitro-aromatics was detected. The enzyme was inactivated by dialysis and was reactivated by the addition of NADH or NADPH. NADPH was the more efficient co-substrate.
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Schackmann, A., Müller, R. Reduction of nitroaromatic compounds by different Pseudomonas species under aerobic conditions. Appl Microbiol Biotechnol 34, 809–813 (1991). https://doi.org/10.1007/BF00169355
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DOI: https://doi.org/10.1007/BF00169355