Summary
A production method for alkaline serine protease with Bacillus licheniformis in a synthetic medium was developed. Employing closed-loop control of oxygen, nitrogen and carbon source the pO2 was held at 5%, the ammonium concentration kept below 1 mM and the glycerol concentration was maintained between 20 and 100 mM. Protease production was monitored by flow injection analysis. Thus, in a fed-batch procedure production could be increased 4.6-fold in comparison to an uncontrolled batch process.
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Giesecke, U.E., Bierbaum, G., Rudde, H. et al. Production of alkaline protease with Bacillus licheniformis in a controlled fed-batch process. Appl Microbiol Biotechnol 35, 720–724 (1991). https://doi.org/10.1007/BF00169884
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DOI: https://doi.org/10.1007/BF00169884