Skip to main content
Log in

Characterization of hybrids obtained by protoplast fusion, between Pachysolen tannophilus and Saccharomyces cerevisiae

  • Applied Genetics and Regulation
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The genes for utilization of xylose were transferred from Pachysolen tannophilus to Saccharomyces cerevisiae. The hybrids resembled the S. cerevisiae parent morphologically and in sugar assimilation. Pulsed field gel electrophoresis showed that the chromosome banding pattern was intermediate between the two parental species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Becher D, Conrad B, Bottcher F (1982) Genetic transfer mediated by isolated nuclei in Saccharomyces cerevisiae. Curr Genet 6:177–180

    Google Scholar 

  • Dixon WJ (ed) BMDP Statistical Software (1981). Dept. Biomathematics, Univ. California, Los Angeles, USA

    Google Scholar 

  • Kreger-van Rij NJW (ed) (1984) The yeasts, a taxonomic study, 3rd Ed. Elsevier, Amsterdam

    Google Scholar 

  • Onishi H, Perry MB (1965) The production of meso-glyceriodo-heptitol by Pichia miso. Can J Microbiol 11:929–934

    Google Scholar 

  • Reynolds NC (1990) Genetic manipulation of yeast strains. Ph. D. Thesis, Greenwhich University, London, UK

    Google Scholar 

  • Skoog K, Hahn-Hagerdahl B (1988) Xylose fermentation. Enzym Microb Technol 10:66–80

    Article  Google Scholar 

  • Spencer JFT, Laud P, Spencer DM (1980) The use of mitochondrial mutants in the isolation of hybrids involving industrial yeast strains. II. Use in the isolation of hybrids obtained by protoplast fusion. Mol Gen Genet 178:651–654

    Google Scholar 

  • Spencer JFT, Spencer DM, Bruce IJ (1989) Yeast genetics, a manual of methods. Springer, Berlin Heidelberg New York

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Correspondence to: H. Heluane

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heluane, H., Spencer, J.F.T., Spencer, D. et al. Characterization of hybrids obtained by protoplast fusion, between Pachysolen tannophilus and Saccharomyces cerevisiae . Appl Microbiol Biotechnol 40, 98–100 (1993). https://doi.org/10.1007/BF00170435

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00170435

Keywords

Navigation