Skip to main content
SpringerLink
Log in

Culture requirements for the production of protease by Aspergillus oryzae in solid state fermentation

  • Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

A number of culture conditions for protease production by Aspergillus oryzae NRRL 2160 on solid substrates were investigated. The pH of the medium and the substrate markedly affected protease production. High protease yield was obtained when the fungus was cultivated for 72–96 h on rice hulls: rice bran (7:3), at an initial pH of 7.0. Maximal protease production was achieved at an initial moisture content of 35–40%, corresponding to a water activity range of 0.982–0.986. Casein and gluten were effective inducers. Polyethylene bags proved to be promising containment systems for solid state cultivation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aidoo KE, Hendry R, Wood JB (1982) Solid substrate fermentations. Adv Appl Microbiol 28:201–237

    Article  CAS  Google Scholar 

  • Anson ML (1938) Estimation of pepsin, typsin, papain, and cathepsin with hemoglobin. J Gen Physiol 22:79–89

    Article  CAS  Google Scholar 

  • Aunstrup K (1980) Proteinases. In: Rose AH (ed) Economic microbiology, vol 5. Academic Press, London, pp 49–113

    Google Scholar 

  • Blakeman JP, McCracken AR, Seaby DA (1988) Changes brought about in solid substrates after fermentations of mixtures of cereals and pulses with Rhizopus oryzae. J Sci Food Agric 45:109–118

    Article  CAS  Google Scholar 

  • Carrizales V, Jaffe W (1986) Solid state fermentation: an appropriate biotechnology for developing countries. Intersciencia 11:9–15

    Google Scholar 

  • Cohen BL, Morris JE, Drucker H (1975) Regulation of two extracellular proteases of Neurospora crassa by induction and by carbon-nitrogen and sulphur-metabolite repression. Arch Biochem Biophys 169:324–330

    Article  CAS  Google Scholar 

  • Considine PJ, Buckley RJ, Griffin TO, Tuohy MG, Coughlan MP (1989) A simple and inexpensive method of solid-state cultivation. Biotechnol Tech 3:85–90

    Article  CAS  Google Scholar 

  • Cuero RG, Smith JE, Lacey J (1985) A novel containment system for laboratory-scale solid particulate fermentations. Biotechnol Lett 7:463–466

    Article  CAS  Google Scholar 

  • Documenta Geigy (1965) Tablas cientificas, 6th edn. Geigy, Basle, Switzerland

    Google Scholar 

  • Dworschack RG, Koepsell HJ, Lagoda AA (1952) Proteolytic enzymes of microorganisms. Evaluation of proteinases produced by molds of the Aspergillus flavus-oryzae group in submerged culture. Arch Biochem Biophys 41:48–60

    Article  CAS  Google Scholar 

  • Fukushima Y, Itoh H, Fukase T, Motai H (1989) Continuous protease production in a carbon-limited chemostat culture by salt tolerant Aspergillus oryzae. Appl Microbiol Biotechnol 30:604–608

    Article  CAS  Google Scholar 

  • Hahn-Hägerdal B (1986) Water activity: a possible external regulator in biotechnical processes. Enzyme Microb Technol 8:322–327

    Article  Google Scholar 

  • Hellmich S, Schauz K (1988) Production of extracellular alkaline and neutral proteases of Ustilago maydis. Exp Mycol 12:223–232

    Article  CAS  Google Scholar 

  • Hesseltine CW (1987) Solid state fermentation — an overview. Int Biodeterior 23:79–89

    Article  CAS  Google Scholar 

  • Keay L (1971) Microbial proteases. Process Biochem 17–21

  • Klapper BF, Jameson DM, Mayer RM (1973a) The purification and properties of an extracellular protease of Aspergillus oryzae NRRL 2160. Biochim Biophys Acta 304:505–512

    Article  CAS  Google Scholar 

  • Klapper BF, Jameson DM, Mayer RM (1973b) Factors affecting the synthesis and release of the extracellular protease of Aspergillus oryzae NRRL 2160. Biochim Biophys Acta 304:513–519

    Article  CAS  Google Scholar 

  • Montes AL (1981) Bromatologia. Universitaria de Buenos Aires, Argentina

    Google Scholar 

  • Nakadai T, Nasuno S (1988) Culture conditions of Aspergillus oryzae for production of enzyme preparation. J Ferment Technol 66:525–533

    Article  CAS  Google Scholar 

  • Narahara H, Koyama Y, Yoshida T, Pichangkura S, Ueda R, Taguchi H (1982) Growth and enzyme production in a solid-state culture of Aspergillus oryzae. J Ferment Technol 60:311–319

    CAS  Google Scholar 

  • Ueno S, Miyama M, Ohashi Y, Izumiya M, Kusaka I (1987) Secretory enzyme production and conidiation of Aspergillus oryzae in submerged liquid culture. Appl Microbiol Biotechnol 26:273–276

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina

    R. A. Battaglino, A. M. R. Pilosof & G. B. Bartholomai

  2. Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina

    R. A. Battaglino & M. Huergo

  3. Members Consejo Nacional de Investigaciónes Cienetíficas y Técnicas de la República Argentina, Argentina

    A. M. R. Pilosof & G. B. Bartholomai

Authors
  1. R. A. Battaglino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Huergo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. R. Pilosof
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. B. Bartholomai
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Offprint requests to: A. M. R. Pilosof

Rights and permissions

Reprints and permissions

About this article

Cite this article

Battaglino, R.A., Huergo, M., Pilosof, A.M.R. et al. Culture requirements for the production of protease by Aspergillus oryzae in solid state fermentation. Appl Microbiol Biotechnol 35, 292–296 (1991). https://doi.org/10.1007/BF00172714

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00172714

Keywords

Search

Navigation