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Characterization of prejunctional purinoceptors on adrenergic nerves of the rat caudal artery

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Summary

The effects of a number of purinoceptor agents on the release of endogenous noradrenaline from the electrically stimulated rat caudal artery were determined. Noradrenaline was quantified by high performance liquid chromatography-electrochemical detection techniques. Both P1-receptor and P2-receptor agonists reduced the release of noradrenaline; the relative order of potency being 2-chloroadenosine > beta, gamma methylene ATP > ATP ≥ adenosine. The adenosine uptake inhibitor S-p-nitrobenzyl-6-thioguanosine potentiated the effects of adenosine but not those of the adenine nucleotides. This suggests that the nucleotides do not need to be converted to adenosine to produce a prejunctional inhibition of the release of noradrenaline. The P1-receptor antagonist 8-(p-sulfophenyl) theophylline reduced the inhibitory effects of both P1- and P2-receptor agonists as did the photolysis of tissues with an intense light source. The findings indicate that prejunctional purinoceptors that mediate an inhibition of the release of noradrenaline from the adrenergic nerves of the caudal artery may not be adequately defined as either P1- or P2-receptors and thus appear to represent a unique receptor. We suggest referring to these receptors as P3-purinoceptors.

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References

  • Brown CM, Burnstock G (1981) The structural conformation of the polyphosphate chain of the ATP molecule is critical for its promotion of prostaglandin biosynthesis. Eur J Pharmacol 69:81–86

    Google Scholar 

  • Brown CM, Collis MG (1983) Adenosine A1-receptor mediated inhibition of nerve stimulation-induced contractions of the rabbit portal vein. Eur J Pharmacol 93:277–282

    Google Scholar 

  • Burnstock G (1978) A basis for distinguishing two types of purinergic receptor. In: Bolis L, Staub RW (eds) Cell membrane receptors for drugs and hormones: A multidisciplinary approach. Rauch Press, New York, pp 107–118

    Google Scholar 

  • Burnstock G (1981) Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol 313:1–35

    Google Scholar 

  • Burnstock G (1983) A comparison of receptors for adenosine and adenine nucleotides. In: Berne RM, Rall TW, Rubio R (eds) Regulatory function of adenosine. Martinus Nijhoff, Boston, pp 49–62

    Google Scholar 

  • Burnstock G (1985) Nervous control of smooth muscle by transmitters, cotransmitters and modulators. Experientia 41:869–874

    Google Scholar 

  • Burnstock G, Buckley NJ (1985) The classification of receptors for adenosine and adenine nucleotides. In: Paton DM (ed) Methods in pharmacology, vol 6, Methods used in adenosine research. Plenum Press, New York, pp 193–212

    Google Scholar 

  • Burnstock G, Wong H (1978) Comparison of the effects of ultraviolet light and purinergic nerve stimulation on the guinea-pig taenia coli. Br J Pharmacol 62:293–302

    Google Scholar 

  • Clanachan AS, Johns A, Paton DM (1977) Presynaptic actions of adenine nucleotides and adenosine on neurotransmission in the rat vas deferens. Neuroscience 2:597–602

    Google Scholar 

  • Enero MA, Saidman BQ (1977) Possible feedback inhibition of noradrenaline release by purine compounds. Naunyn-Schmiedeberg's Arch Pharmacol 297:39–46

    Google Scholar 

  • Fedan JS, Hogaboom GK, Westfall DP, O'Donnell JP (1982) Comparison of contractions of the smooth muscle of the guinea-pig vas deferens induced by ATP and related nucleotides. Eur J Pharmacol 81:193–204

    Google Scholar 

  • Fedan JS, Hogaboom GK, O'Donnell JP, Westfall DP (1985) Use of photoaffinity labels as P2-purinoceptor antagonists. In: Paton DM (ed) Methods in pharmacology, vol 6, Methods used in adenosine research. Plenum Press, New York, pp 279–292

    Google Scholar 

  • Fredholm BB, Fried G, Hedqvist P (1982) Origin of adenosine released from rat vas deferens by nerve stimulation. Eur J Pharmacol 79:233–243

    Google Scholar 

  • Fredholm BB, Hedqvist P (1980) Modulation of neurotransmission by purine nucleotides and nucleosides. Biochem Pharmacol 29:1635–1643

    Google Scholar 

  • Frew R, Lundy PM (1986) Arylazido aminopropionyl ATP (ANAPP3): interaction with adenosine receptors in longitudinal smooth muscle of the guinea-pig ileum. Eur J Pharmacol 123:395–400

    Google Scholar 

  • Fujiwara S, Kassell NF, Sasaki T, Yamashita M, Zuccarello M (1986) Presynaptic inhibitory action of adenosine on neuromuscular transmission in the canine cavernous carotid artery. Stroke 17:312–317

    Google Scholar 

  • Hedqvist P, Fredholm BB (1976) Effects of adenosine on adrenergic neurotransmission; prejunctional inhibition and postjunctional enhancement. Naunyn-Schmiedeberg's Arch Pharmacol 293:217–223

    Google Scholar 

  • Hom GJ, Lokhandwala MF (1981) Presynaptic inhibition of vascular sympathetic neurotransmission by adenosine. Eur J Pharmacol 69:101–106

    Google Scholar 

  • Husted SE, Nedergaard OA (1981) Inhibition of adrenergic neuroeffector transmission in rabbit main pulmonary artery and aorta by adenosine and adenine nucleotides. Acta Pharmacol Toxicol 49:334–353

    Google Scholar 

  • Husted SE, Nedergaard OA (1985) Dual inhibitory action of ATP on adrenergic neuroeffector transmission in rabbit pulmonary artery. Acta Pharmacol Toxicol 57:204–213

    Google Scholar 

  • Kenakin TP, Leighton HJ (1985) The pharmacology estimation of potencies of agonists and antagonists in the classification of adenosine receptors. In: Paton DM (ed) Methods in pharmacology, vol. 6, Methods used in adenosine research. Plenum Press, New York, pp 213–237

    Google Scholar 

  • Kennedy C, Burnstock G (1984) Evidence for an inhibitory prejunctional P1-purinoceptor in the rat portal vein with characteristics of the A2 rather than of the A1 subtype. Eur J Pharmacol 100:363–368

    Google Scholar 

  • Khan MT, Malik KU (1980) Inhibitory effect of adenosine and adenine nucleotides on potassium-evoked efflux of [3H]-noradrenaline from the rat isolated heart: lack of relationship to prostaglandins. J Pharmacol Exp Ther 68:551–561

    Google Scholar 

  • Langer SZ (1977) Presynaptic receptors and their role in the regulation of transmitter release. Br J Pharmacol 60:481–497

    Google Scholar 

  • Lukacsko P, Blumberg A (1982) Modulation of the vasoconstrictor response to adrenergic stimulation by nucleosides and nucleotides. J Pharmacol Exp Ther 222:344–349

    Google Scholar 

  • Maguire MH, Satchell DG (1979) The contribution of adenosine to the inhibitory actions of adenine nucleotides on the guinea-pig taenia coli: studies with phosphate-modified adenine nucleotides analogs and dipyridamole. J Pharmacol Exp Ther 211:626–631

    Google Scholar 

  • Moylan RD, Westfall TC (1979) Effect of adenosine on adrenergic neurotransmission in the superfused rat portal vein. Blood Vessels 16:302–310

    Google Scholar 

  • Paterson ARP, Jakobs ES, Harley ER, Fu NW, Robins MJ, Cass CE (1983) Inhibition of nucleoside transport. In: Berne RM, Rall TW, Rubio R (eds) Regulatory function of adenosine. Martinus Nijhoff, Boston, pp 203–220

    Google Scholar 

  • Paton DM (1979) Presynaptic inhibition of adrenergic neurotransmission by adenine nucleotides and adenosine. In: Baer H, Drummond GI (eds) Physiological and regulatory functions of adenosine and adenine nucleotides. Raven Press, New York, pp 69–77

    Google Scholar 

  • Paton DM (1981) Presynaptic neuromodulation mediated by purinergic receptors. In: Burnstock G (ed) Purinergic receptors. Chapman and Hall, London, p 199–219

    Google Scholar 

  • Shinozuka K, Bjur RA, Westfall DP (1987) Effect of 2-chloroadenosine on release of endogenous norepinephrine from sympathetic nerves of the rat caudal artery. Fed Proc 46:554

    Google Scholar 

  • Starke K (1977) Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol 77:1–24

    Google Scholar 

  • Stjärne L, Astrand P (1985) Relative pre- and postjunctional roles of noradrenaline and adenosine 5′-triphosphate as neurotransmitters of the sympathetic nerves of guinea-pig and mouse vas deferens. Neuroscience 14:929–946

    Google Scholar 

  • Su C (1978) Purinergic inhibition of adrenergic transmission in rabbit blood vessels. J Pharmacol Exp Ther 204:351–361

    Google Scholar 

  • Su C (1983) Purinergic neurotransmission and neuromodulation. Pharmacol Rev 23:397–411

    Google Scholar 

  • Taylor DA, Wiese S, Faison EP, Yarbrough GG (1983) Pharmacological characterization of purinergic receptors in rat vas deferens. J Pharmacol Exp Ther 224:40–45

    Google Scholar 

  • Verhaeghe RH, Vanhoutte PM, Shepherd JT (1977) Inhibition of sympathetic neurotransmission in canine blood vessels by adenosine and adenine nucleotides. Circ Res 40:208–215

    Google Scholar 

  • Wakade AR, Wakade TD (1978) Inhibition of norepinephrine release by adenosine. J Physiol 282:35–49

    Google Scholar 

  • Webster DR, Boston GD, Holford NHG, Paton DM (1986) Relationship of metabolism of 2′,3′- and 5′-adenine nucleotides to presynaptic inhibition of transmitter release in rat vas deferens. Naunyn-Schmiedeberg's Arch Pharmacol 333:163–167

    Google Scholar 

  • Westfall DP, Shinozuka K, Bjur RA (1987) Effects of adenyl purines on electrically evoked norepinephrine release in rat caudal artery. Pharmacologist 29:171

    Google Scholar 

  • Westfall TC (1977) Local regulation of adrenergic neurotransmission. Physiol Rev 57:659–728

    Google Scholar 

  • Yamamoto R, Cline WH Jr (1987) Release of endogenous NE from mesenteric vasculature of WKY and SHR in response to PNS. J Pharmacol Exp Ther 241:826–832

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, University of Nevada School of Medicine, 89557, Reno, Nevada, USA

    Kazumasa Shinozuka, Richard A. Bjur & David P. Westfall

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  1. Kazumasa Shinozuka
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  2. Richard A. Bjur
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  3. David P. Westfall
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Supported by grants from the American Heart Association, the Bently Nevada Corp. and NIH Grant HL38126

Send offprint requests to D. P. Westfall at the above address

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Shinozuka, K., Bjur, R.A. & Westfall, D.P. Characterization of prejunctional purinoceptors on adrenergic nerves of the rat caudal artery. Naunyn-Schmiedeberg's Arch Pharmacol 338, 221–227 (1988). https://doi.org/10.1007/BF00173391

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  • DOI: https://doi.org/10.1007/BF00173391

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