Summary
The localization of arginine-vasopressin in the endothelial cells of rat pulmonary artery was investigated by immunocytochemistry at the light and electron microscopic levels. The immunogold silver staining method was used for light microscopy of sheets of endothelium, removed from the artery, and the pre-embedding peroxidase-antiperoxidase technique was used for electron microscopy of cross sections of the artery. With both of the methods used, numerous vasopressin-positive endothelial cells were observed. None of the subendothelial elements showed labelling for vasopressin. The results are discussed in terms of the involvement of the endothelium in local control of the pulmonary circulation.
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References
Altura BM, Altura BT (1977) Vascular smooth muscles and neurohypophysial hormones. Fed Proc, Fed Am Soc Exp Biol 36:1853–1860
Bianch C, Gutkowska J, Thibault G, Garcia R, Genest J, Cantin M (1985) Radioautographic localization of 125I-atrial natriuretic factor (ANF) in rat tissue. Histochemistry 83:441–452
Burnstock G (1988) Regulation of local blood flow by neurohumoral substances released from perivascular nerves and endothelial cells. Acta Physiol Scand 133 [Suppl 571]:53–59
Burnstock B, Lincoln J, Fehér E, Hopwood AM, Kirkpatrick K, Milner P, Ralevic V (1988) Serotonin is localized in endothelial cells of coronary arteries and released during hypoxia: a possible new mechanism for hypoxia-induced vasodilatation of the rat heart. Experientia 44:705–707
Caldwell PRB, Seegal BC, Hsu KS, Das M, Soffer RL (1976) Angiotensin II converting enzyme: vascular endothelial localization. Science 191:1050–1051
Cowley AW, Jr, Switzer SJ, Guinn MM (1980) Evidence and quantification of the vasopressin arterial pressure control system in dog. Circ Res 46:58–67
Crofton JT, Shade L, Shade RE, Lee-Kwon WH, Mouning M, Sawyer WH (1979) The importance of vasopressin in the development and maintenance of DOC-salt hypertension in the rat. Hypertension 1:31–38
Furchgott RF, Zawadzki JV (1980) The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288:373–376
Holgate CS, Jackson P, Cowen PN, Bird C (1983) Immunogoldsilver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31:938–944
Junod AF (1972) Uptake, metabolism and efflux of 14C-5-hydroxytryptamine in isolated perfused rat lungs. J Pharmacol Exp Ther 183:341–355
Katusic ZS, Shepherd JT, Vanhoutte PH (1984a) Arginine vasopressin induces endothelium-dependent relaxation of canine basilar and coronary arteries. Fed Proc [Abstracts] 34:1084
Katusic ZS, Shepherd JT, Vanhoutte PH (1984b) Vasopressin causes endothelium-dependent relaxation of the canine basilar artery. Circ Res 55:575–579
Kay JM (1983) Pulmonary vasculature and nerves. Comparative morphological features of the pulmonary vasculature in mammals. Am Rev Respir Dis 128:553–557
Lim ATW, Lolait SJ, Barlow JW, Autelitano DJ, Toh BM, Boublik J, Abraham CI, Funder JW (1984) Immunoreactive arginine-vasopressin in Brattleboro rat ovary. Nature 310:61–64
Lincoln J, Burnstock G (1990) Neural-endothelial interactions in control of local blood flow. In: Warren J (ed) The endothelium: an introduction to current research. Wiley-Liss, New York, pp 21–32
Lincoln J, Loesch A, Burnstock G (1990) Localization of vasopressin, serotonin and angiotensin II in endothelial cells of the renal and mesenteric arteries of the rat. Cell Tissue Res 259:341–344
Loesch A, Burnstock G (1988) Ultrastructural localization of serotonin and substance P in vascular endothelial cells of rat femoral and mesenteric arteries. Anat Embryol 178:137–142
McLean JR (1986) Pulmonary vascular innervation. In: Bergofsky EM (ed) Abnormal Pulmonary Circulation. Churchill-Livingstone, New York, pp 27–81
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixation. A new fixative for immuno-electron microscopy. J Histochem Cytochem 22:1077–1083
Milner P, Ralevic V, Hopwood AM, Feher E, Lincoln J, Kirkpatrick KA, Burnstock G (1989). Ultrastructural localization of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia. Experientia 45:121–125
Mohring J, Mohring B, Pelri M, Haack D (1977) Vasopressin role of ADH in the pathogenesis of malignant DIC hypertension. Am J Physiol 232:F260-F269
Parnavelas JG, Kelly W, Burnstock G (1985) Ultrastructural localization of choline acetyltransferase in vascular endothelial cells in rat brain. Nature 316:724–725
Poole JCF, Sanders AG, Florey MW (1958) The regeneration of aortic endothelium. J Pathol Bact 75:133–143
Roche E, Silva M, Jr, Rosenberg M (1969) The release of vasopressin in response to haemorrhage and its role in the mechanism of blood pressure regulation. J Physiol (Lond) 202:535–557
Ryan JW (1984) Metabolism of angiotensin I and bradykinin by endothelial cells. In: Jaffe EA (ed) Biology of endothelial cells. Martinus Nijhoff, Lancaster, pp 317–329
Ryan JW, Ryan US, Scholtz DR, Whitaker C, Chong A, Dorev FE (1975) Subcellular localization of pulmonary angiotensin-converting enzyme (Kinase II). Biochem J 146:497–499
Sundler F, Håkanson R, Luts A, Uddman R (1988) Vascular innervation in the respiratory tract with special reference to neuropeptides. In: Burnstock G, Griffith SG (eds) Nonadrenergic innervation of blood vessels, vol II, Regional innervation. CRC Press, Boca Raton, Florida, pp 95–106
Tomlinson A, Van Vlijmen H, Loesch A, Burnstock G (1991) An immunohistochemical study of endothelial cell heterogeneity in the rat: observations in “en face” Häutchen preparations. Cell Tissue Res 263:173–181
Toshimori H, Nakazato M, Toshimori K, Asai J, Matsukura S, Qura Ch, Matsuo H (1988) Distribution of atrial natriuretic peptide (ANP)-containing cells in the heart and pulmonary vein. Immunocytochemical study and radioimmunoassay. Cell Tissue Res 251:541–546
Vandesande F, Dierickx K (1975) Identification of the vasopressin-producing and oxytocin-producing neurons in the hypothalamic magnocellular neuronal system of the rat. Cell Tissue Res 164:153–162
Vanhoutte PM, Miller VM (1985) Heterogeneity of endotheliumdependent responses in mammalian blood vessel. J Cardiovasc Pharmacol [Suppl] 3:S12-S23
Wharton J, Haworth SG, Polak JM (1988) Postnatal development of the innervation and paraganglia in the porcine pulmonary arterial bed. J Pathol 154:19–27
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Loesch, A., Tomlinson, A. & Burnstock, G. Localization of arginine-vasopressin in endothelial cells of rat pulmonary artery. Anat Embryol 183, 129–134 (1991). https://doi.org/10.1007/BF00174393
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DOI: https://doi.org/10.1007/BF00174393