Summary
This paper presents results of experiments on the influence of O2 and substrate (pollutant) concentration on the overall reaction rate of a trickle-bed reactor used for biological waste gas purification. The biocatalyst was a pollutant-specific bacterial monoculture fixed on porous glass carriers. The conversion of acetone and propionaldehyde, as model pollutants that are easily soluble in water, was measured. Under constant hydrodynamic conditions (gas and liquid flow rates) the inlet pollutant concentration was varied. The O2 partial pressure in the model gas was increased to investigate the influence of O2 supply on pollutant conversion. At higher pollutant concentrations (>117 mg acetone.m-3 gas and > 150 mg propionaldehyde.m-3 gas) higher concentrations of dissolved O2 led to a significant rise in the maximum degradation capacity of the reactor. This maximum reaction rate was independent of the pollutant mass flow. It seems that the diffusion of O2 in the biofilm is rate-determining. The reaction rate at lower inlet concentrations was not affected by the improved O2 supply. Here the external mass transfer through the liquid film limits the reaction rate and the maximum separation efficiency of about 80% at a residence time of 1.2s (space velocity 3000h-1) is achieved.
Similar content being viewed by others
References
Chang HN, Moo-Young M (1988) Estimation of oxygen penetration depth in immobilized cells. Appl Microbiol Biotechnol 29:107–112
Erhardt HM, Rehm H-J (1985) Phenol degradation by microorganisms adsorbed on activated carbon. Appl Microbiol Biotechnol 21:32–36
Fischer K, Bardtke D, Eitner D, Homans WJ, Janson O, Kohler H, Sabo F, Schirz St (1990) Biologische Abluftreinigung. Expert Verlag, Ehningen/Böblingen
Howell JA, Atkinson B (1976) Influence of oxygen and substrate concentration on the ideal film thickness and the maximum overall substrate uptake rate in microbial film fermentors. Biotechnol Bioeng 18:15–35
Kirchner K, Hauk G, Rehm H-J (1987) Exhaust gas purification using immobilized monocultures. Appl Microbiol Biotechnol 26:579–587
Kirchner K, Schlachter U, Rehm H-J (1989) Biological purification of exhaust air using fixed bacterial monocultures. Appl Microbiol Biotechnol 31:629–632
Ottengraf SP (1986) Exhaust gas purification. In: Rehm H-J, Reed G (eds) Biotechnology, vol 8. VCH, Weinheim, pp 425–452
VDI-Berichte 735 (1989) Biologische Abgasreinigung. VDI, Düsseldorf
VDI-Richtlinie 3478 (1985) Biologische Abluftreinigung, Biowäscher. VDI, Düsseldorf
VDI-Richtlinie 3477 (1989) Biologische Abluftreinigung, Biofilter. VDI, Düsseldorf
Wagner K, Hempel DC (1988) Biodegradation by immobilized bacteria in an airlift-loop-reactor — influence of biofilm diffusion limitation. Biotechnol Bioeng 31:559–566
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kirchner, K., Wagner, S. & Rehm, HJ. Exhaust gas purification using biocatalysts (fixed bacteria monocultures) — the influence of biofilm diffusion rate (O2) on the overall reaction rate. Appl Microbiol Biotechnol 37, 277–279 (1992). https://doi.org/10.1007/BF00178184
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00178184