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Characterization of a bacterial xylanase resistant to repression by glucose and xylose

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Summary

Bacillus subtilis CD4, when grown in nutrient broth or minimal medium in presence of xylan, produced extracellular xylanase that hydrolyzed xylan optimally at pH 5. The enzyme was induced by xylan, xylose and glucose. Addition of xylose or glucose in xylan containing medium did not affect enzyme production. The structural gene encoding xylanase was cloned and expressed in E. coli. The recombinant enzyme exhibited similar properties like that of native enzyme including resistance to repression by xylose and glucose.

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References

  • Berenger, J.F., Friscon, C., Gliarde, J.B. and Cruezer, N. (1985) Can. J. Microbiol. 31, 635–643.

    Google Scholar 

  • Bernier Jr., R., Driguez, H. and Desrochers, N. (1983) Gene 26, 59–65.

    Google Scholar 

  • Biely, P. (1985) Trends Biotechnol. 3, 286–290.

    Google Scholar 

  • Fisher, S.H. and Sonenshein, A.L. (1991) Annu. Rev. Microbiol. 45:107–35.

    Google Scholar 

  • Fisher, S.H. and Magasanik, B. (1984) J. Bacteriol. 158, 379–82.

    Google Scholar 

  • Gilbert, H.J., Sullivan, D.A., Jenkins, G., Kellett, L.E., Minton, N.P. and Hall, J. (1988) J. Gen. Microbiol, 134, 3239–3247.

    Google Scholar 

  • Klier, A.F. and Rapoport, G. (1988) Ann. Rev. Microbiol, 42:65–95.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory.

  • Miller, G.L., Blum, R., Glennon, W.E. and Burton, A.L. (1960) Anal. Biochem. 2, 127–132.

    Google Scholar 

  • Miller, J.H. (1972) Experiments in Molecular genetics, CSHL, CSH, NY.

    Google Scholar 

  • Minton, N.P., Atkinson, T. and Sherwood, R.F. (1986) J. Bacteriol. 156, 1222–1227.

    CAS  PubMed  Google Scholar 

  • Panbangred, W., Kondo, T., Negoro, S., Shinmyo, A. and Okada, H. (1983) Mol. Gen. Genet. 192, 335–341.

    Google Scholar 

  • Srivastava, R., Bharti, R. and Srivastava, A.K. (1990) Biotechnol. Lett. 12, 541–545.

    Google Scholar 

  • Srivastava, R., Ali, S.S. and Srivastava, B.S. (1991) FEMS Microbiol. Lett. 78 (2/3) 201–206.

    Google Scholar 

  • Wood, P.J., Erfle, J.D. and Teather, R.M. (1988) In Methods in Enzymology, 160, 63–70.

    Google Scholar 

  • Woodward, J. (1984) Xylanases: Functions, Properties and Applications. Top. Enzyme Ferment. Biotechnol. 8:9–30.

    Google Scholar 

  • Yang, R.C.A., McKenzie, C.R., Bilous, D., Seligy, V.L. and Narang, S.A. (1988) Appl. Environ. Microbiol. 54, 1023–1029.

    Google Scholar 

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Authors and Affiliations

  1. Division of Microbial Genetics, Central Drug Research Institute, 226001, Lucknow, India

    Ranjana Srivastava & Alok K. Srivastava

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  1. Ranjana Srivastava
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  2. Alok K. Srivastava
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Srivastava, R., Srivastava, A.K. Characterization of a bacterial xylanase resistant to repression by glucose and xylose. Biotechnol Lett 15, 847–852 (1993). https://doi.org/10.1007/BF00180153

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