Summary
Batch propionic acid fermentations by Propionibacterium acidipropionici with lactose, glucose, and lactate as the carbon source were studied. In addition to propionic acid, acetic acid, succinic acid and CO2 were also formed from lactose or glucose. However, succinic acid was not produced in a significant amount when lactate was the growth substrate. Compared to fermentations with lactose or glucose at the same pH, lactate gave a higher propionic acid yield, lower cell yield, and lower specific growth rate. The specific fermentation or propionic acid production rate from lactate was, however, higher than that from lactose. Since about equimolar acid products would be formed from lactate, the reactor pH remained relatively unchanged throughout the fermentation and would be easier to control when lactate was the growth substrate. Therefore, lactate would be a preferred substrate over lactose and glucose for propionic acid production using continuous, immobilized cell bioreactors.
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References
Blanc P, Goma G (1987a) Propionic acid fermentation: improvement of performances by coupling continuous fermentation and ultrafiltration. Bioprocess Eng 2:137–139
Blanc P, Goma G (1987b) Kinetics of inhibition in propionic acid fermentation. Bioprocess Eng 2:175–179
Bodie EA, Anderson TM, Goodman N, Schwartz RD (1987) Propionic acid fermentation of ultra-high-temperature sterilized whey using mono- and mixed-cultures. Appl Microbiol Biotechnol 25:434–437
Border PM, Kierstan MPJ, Plastow GS (1987) Production of propionic acid by mixed bacterial fermentation. Biotechnol Lett 9:843–848
Boyaval P, Corre C (1987) Continuous fermentation of sweet whey permeate for propionic acid production in a CSTR with UF recycle. Biotechnol Lett 9:801–806
Cavin JF, Saint C, Divies C (1985) Continuous production of Emmental cheese flavours and propionic acid starters by immobilized cells of a propionic acid bacterium. Biotechnol Lett 7:821–826
Clausen EC, Gaddy JL (1981) Fermentation of biomass into acetic and propionic acids with Propionibacterium acidipropionici. In: Moo-Young M, Robinson CW (eds) Advances in biotechnology, vol II. Pergamon, Toronto, pp 63–69
Crespo JPSG, Moura MJ, Carrondo MJT (1990) Some engineering parameters for propionic acid fermentation coupled with ultrafiltration. Appl Biochem Biotechnol 24/25:613–625
Datta R (1981) Acidogenic fermentation of corn stover. Biotechnol Bioeng 23:61–77
Emde R, Schink B (1990) Enhanced propionate formation by Propionibacterium freudenreichii subsp. freudenreichii in a three-electrode amperometric culture system. Appl Environ Microbiol 56:2771–2776
Hendricks B, Korus RA, Heimsch RC (1985) Propionic acid production by bacterial fermentation. Biotechnol Bioeng Symp 15:241–245
Hettinga DH, Reinbold GW (1972a) The propionic-acid bacteria —a review. I. Growth. J Milk Food Technol 35:295–301
Hettinga DH, Reinbold GW (1972b) The propionic-acid bacteria —a review. II. Metabolism. J Milk Food Technol 35:358–372
Hettinga DH, Reinbold GW (1972c) The propionic-acid bacteria —a review. III. Miscellaneous metabolic activities. J Milk Food Technol 35:436–447
Hsu ST, Yang ST (1991) Propionic acid fermentation of lactose by Propionibacterium acidipropionici: effects of pH. Biotechnol Bioeng 38:571–578
Ibragimova SI, Neronova NM, Rabotbova IL (1969) Kinetics of growth inhibition in Propionibacterium shermanii by hydrogen and hydroxyl ions. Mikrobiologiya 38:933–939
Langsrud T, Reinbold GW (1973) Flavor development and microbiology of Swiss cheese — a review. III. Ripening and flavor production. J Milk Food Technol 36:593–609
Lee IH, Fredrickson AG, Tsuchiya HM (1974) Diauxic growth of Propionibacterium shermanii. Appl Microbiol 28:831–835
Lewis VP (1991) A novel extractive fermentation process for propionic acid production from lactose using an immobilized cell bioreactor. M.S. thesis, Ohio State University, Columbus
Lewis VP, Yang ST (1992) A novel extractive fermentation process for propionic acid production from whey lactose. Biotechnol Prog 8:104–110
Lewis VP, Yang ST (1992) Continuous propionic acid fermentation by immobilized Propionibacterium acidipropionici in a novel packed-bed bioreactor. Biotechnol Bioeng in press
Liu JAP, Moon NJ (1982) Commensalistic interaction between Lactobacillus acidophilus and Propionibacterium shermanii. Appl Environ Microbiol 44:715–722
Neronova NM, Ibragimova SI, Ierusalimskii ND (1967) Effect of the propionate concentration on the specific growth rate of Propionibacterium shermanii. Mikrobiologiya 36:404–409
O'Brien DJ, Panzer CC, Eisele WP (1990) Biological production of acrylic acid from cheese whey by resting cells of Clostridium propionicum. Biotechnol Prog 6:237–242
Playne MJ (1985) Propionic and butyric acids. In: Moo-Young M (ed) Comprehensive biotechnology, vol 3. Pergamon, New York, pp 731–759
Rehberger TG, Glatz BA (1990) Characterization of Propionibacterium plasmids. Appl Environ Microbiol 56:864–871
Thompson TE, Conrad R, Zeikus JG (1984) Regulation of carbon and electron flow in Propionispira arboris: physiological function of hydrogenase and its role in homopropionate formation. FEMS Microbiol Lett 22:265–271
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Correspondence to: S. T. Yang
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Lewis, V.P., Yang, ST. Propionic acid fermentation by Propionibacterium acidipropionici: effect of growth substrate. Appl Microbiol Biotechnol 37, 437–442 (1992). https://doi.org/10.1007/BF00180964
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DOI: https://doi.org/10.1007/BF00180964